Lecture 5

Question 1

Environmental Factors of cell growth

Answer 1

Temp, and nutrient availability

Question 2

Which divisome component forms a ring around the center of the dividing cell

Answer 2

FtsZ

Question 3

Which divisome component connects the FstZ ring to the cytoplasmic membrane (the anchor)

Answer 3

ZipA

Question 4

Which divisome component attracts other Fts protein to the divisome (the Recruiter)

Answer 4

FtsA

Question 5

Which divisome component is involved in peptidoglycan synthesis

Answer 5

Ftsl

Question 6

Which protein forms the cellular cytoskeleton

Answer 6

MreB

Question 7

MreB protein is absent in which type of bacteria

Answer 7

Coccoid bacteria

Question 8

MreB protein is present in which type of bacteria

Answer 8

rod-shaped bacteria

Question 9

Which phase is the healthiest state of cells for study

Answer 9

The exponential phase

Question 10

What is cryptic growth

Question 11

In which phase is the growth rate zero (also when cryptic growth occurs)

Answer 11

stationary phase

Question 12

In which phase is the growth rate negative

Answer 12

Death phase

Question 13

What is the advantage of a continuous culture (Chemostat)

Answer 13

Keeps the cells in the exponential phase by controlling the dilution rate and nutrient concentration

Question 14

….. measures the microbial growth by counting the number of cells (dead and alive) on a grid

Answer 14

(Microscopic count) cell counts

Question 15

….. measures the microbial growth by counting viable cells in a agar plate

Answer 15

(viable count) Plate count

Question 16

Describe the great plate anomaly

Answer 16

Even when cells are taken from the same sample microscopic counts will always be higher than viable counts

Question 17

…. is a simple/non-destructive method of measuring cell mass and number

Answer 17

Turbidimetric method

Question 18

What type of cells live in very cold or very hot environments

Answer 18

extremophiles

Question 19

What type of extremophile has an optimal temp of <15ºC and is slow growing

Answer 19

psychrophile

Question 20

Snow algae and Seaice diatoms are examples of what type of extremophiles

Answer 20

psychrophiles

Question 21

What type of molecular adaptation is a “cold-shock” protein and is referred to as antifreeze for the cell

Answer 21

cryoprotectant

Question 22

What type of molecular adaptation increases fluidity at low temps as well as unsaturation/short-chain FAs

Answer 22

Cytoplasmic membrane

Question 23

What type of extremophile has an optimal temp of <45ºC

Answer 23

Thermophiles

Question 24

What type of extremophile has an optimal temp of <15ºC

Question 25

Boiling Springs Bacteria, Hot Springs Cyanobacteria, Hydrothermal Vent Microbes are all examples of

Answer 25

Heat attracted extremophiles

Question 26

What type of molecular adaptation increases stability at high temps as well as saturation/long-chain FAs

Answer 26

cytoplasmic membrane