Lecture 4

Question 1

This is from a peripheral blood smear:

1. What is the arrow pointing to?
2. What causes these to develop?

Answer 1

1. Heinz body
2. Oxidative damage to the RBCs (onions, garlic, etc.)

Question 2

This is from a peripheral blood smear:

1. Cell type at end of YELLOW arrow?
2. Cell type at end of GREEN arrow?
3. Cell type at end of BLACK arrow?

Answer 2

1. Yellow = eccentrocyte
2. Green = ghost cell
3. Black = heinz body

Question 3

What is an eccentrocyte?

Answer 3

A RBC where all the hemoglobin has been pushed to one side of the cell.

Question 4

How would the following affect erythrocytes:

1. Denaturation of hemoglobin?
2. Oxidation and cross-linking of membrane proteins?
3. Oxidation of hemoglobin iron?

Answer 4

1. Heinz body formation
2. Eccentrocyte formation
3. Methemoglobin formation

Question 5

What does the hemoglobin pad detect in a urinarlysis?

Answer 5

Heme

(Hemoglobin, hematuria, myoglobin)

Question 6

1. Which cell type is at the end of the GREEN arrow?
2. Which cell type is at the end of the BLACK arrow?

Answer 6

1. Green = nucleated RBC
2. Black = reticulocyte (polychromatophil)

Question 7

New methylene blue stain allows one to identify reticulocytes in a blood smear. What in the cell does the stain highlight?

Answer 7

mRNA

Question 8

What are 3 common plants that are oxidative agents in veterinary medicine?

Answer 8

Onions

Garlic

Red maple leaves (horses)

Question 9

What drugs are oxidative agents in veterinary medicine?

Answer 9

Acetaminophen

Propofol

Hydrogen peroxide

Benzocaine

Nitrates (ruminants)

Vitamin K1 & K3

Naphthalane (moth balls)

Thiols