Lecture 3D Flashcards
Types of one-carbon metabolism
Acetogenesis, and Methanogenesis
Net reaction of Acetogenesis
4H2 + H+ +2HCO3- -> Acetate + 4H2O
Overview of Acetognesis
CO2 is reduced using hydrogen to form acetyl CoA
what is the intermediates needed to form acetyl CoA
Formate and Tetrahydrofolate (THF)
The organism where acetognesis is observed
Acetobacterium woodie
Other name for acetogenesis pathway
Wood-ljungdahl pathway
The complex that generates IMF
Rnf complex
How does acetogens get energy?
Ion motive force (movement of sodium ions)
what is used in rnf complex as electron donor?
Reduced ferrodoxin
accepts two electrons at a time and donates one of these electrons to a higher-potential electron acceptor
Flavin
Methanogenesis is catalyzed by a group of strictly anaerobic Archaea called the
methanogens
in methanogenesis, what is the major patthway of this process?
Reduction of CO2 by H2 to form Methane
Steps of methanogenesis
Activation of CO2 -> Transfer of formyl -> Transfer of methyl -> Reduction of methyl-C -> regeneration of COM and CoB
Activates the CO2 in methanogenesis
Methanofuran-containing enzyme
produces aldolase and two molecules of lactate
Homofermentative lactic acid bacteria
does not have aldolase so they oxidzie G6P
Heterofermentative bacteria
What is converted to lactic acid by heterofermenters
Triose phosphate
Distinct features of Entner-Duodoroff pathway?
- Occurs in prokaryotes
- Uses 6-Phosphogluconate dehydratase
- 2-ketoo-3-deoxyphosphogluconate
a situation in which two different microbes cooperate to degrade a substance that neither can degrade alone
Syntrophy
Example of syntrophy
Methanogen and Pelotomaculum
In synotrophy, what does Pelotomaculum convert?
converting organic matter into by products like hydrogen and acetate
