Lecture 3 - Enzymes Flashcards
organisms should be able to
self replicate
catalyse chemical reactions
primary alcohol + NAD+ —>
aldehyde + NADH + H+
free energy change
amount energy available to do work
activation energy
energy needed to change states
6 enzyme classifications
oxidoreductases transfereasres/ kinase hydrolases lyases isomerases ligase
oxidoreductases
transfer electrons - alcohol dehydrogenase
transferases/ kinase
group transfer reactions
hydrolases
hydrolysis- transfer of functional groups
lyases
cleavage c-c c-o c-n decarboxylase + deaminase
isomerases
transer groups to form isomers
ligase
formation of bonds coupled ATP
RNAse A
catalyses the hydrolysis of RNA
reaction only takes place at extreme pH w/out enzymes
position
enzyme assay
measures enzyme activity
IU amount of enzyme
amount of enzyme causing transformations
Kcat
molecules of substrates no. that can form product= turnover number
