lecture 3 Flashcards
how does UBL attach to proteins
covalently
describe the way in which UBLs are attached
carboxyl terminus plus the lysine side chain form a isopeptide bond by a condensation reaction.
what does the attachment of UBL do
1)control protein levels 2)changes in protein localisation 3)alter protein function 4)control gene transcription.
describe the cycle in which UBL reaches the target protein.
E1 charges UBL then transferred to E2. E3 ligases transfer the UBL to the target protein.E4 elongates the UBL chain.
what removes the UBL
deubiquitinases
what are the 2 DUB families
thiol proteases and metalloproteases
What is the role of E1 in Ub conjugation
UBA1 which is an E1 and UBA6 have specific E2 partners.
what E1 is found in FAT10ylation
UBA6
what is the function of E2 in Ub conjugation
has a UBC catalytic domain.
which two E2 are large
BIRC6 and UBE2O
in E2 what modulates the UBC activity and function
N and C terminal extensions
which two E3 ligases are known
28 HECT and 14 RBR
which two linkages are best described
K48 AND 63
what does DUB regulation promote
rescues of proteins from proteolytic degradation and restores function to modified proteins.
name 2 ubiquitin related cell surface proteins
EGFR and HER2