LECTURE 3 Flashcards
what can occur after electrophoresis
take fragments and put them together to make your recombinant dna (dna ligase)
how do you create recombinant dna
2 ways:
- fragment isolation using electrophoresis
- fragmenting dna you want doubles of
a common technique to separate different fragments of cut dna so that they can be isolated is ___________. this technique takes advantage of the negative charge on dna by using an electrical field to separate dna molecules.
electrophoresis
what are the steps of electrophoresis
- 3 restriction enzymes
- load fragments into gel
- fragments move based on side
dna libraries are collections of recombinant dna molecules that can be stubly maintained and replicated, via ___________, in a suitable host organism.
cloning vectors
main function of pcr?
allow a way to make more copies of a portion of dna
what are 3 things we can do regarding gene editing
change a genes sequences
inactivate a gene
interfere with the expression of the gene in a cell
__________ mimics dna replication and an individual reaction is cycled through a series of steps in which each step is analogous to a step in dna replication
pcr
during pcr _________ is when you use heat to separate strands of double stranded dna _______ is wehen primers provide the 3’ OH required for elongation by dna polymerase, and ________ is the last step when the dna polymerase makes new dna
denaturation
appealing
synthesis
who is using dna fingerprinting
law enforcement
why do we have to use reverse transcriptase in order to complete PCR to diagnosis the disease COVID-19?
only dna can be copied
how does CRISPR/cas9 work?
cutting dna and then dna repair
what is one of the limitations of using CRISPR within humans?
cant mature cells in a big quantity
A ______________ has a normal allele replaced with an allele that has a specific genetic alteration (i.e. a mutation)
gene knockin
A ______________ is one that has had a gene inactivated so that the function of the gene is lost
gene knockout
