Lecture 2: embryogeneses Flashcards

1
Q

time it takes for a oocyte to degenerate

A

24 hours

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2
Q

time it takes for a sperm to degenerate

A

48 hours

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3
Q

structures released during ovulation

A
  • secondary oocyte
  • corona radiata
  • zona pellicida
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4
Q

sequence of events involved in fertilization (5)

A
  1. penetration of corona radiata
  2. penetration of zona pellicida
  3. fusion of plasma membranes
    - zona reaction: doesn’t allow another sperm to fertilize the oocyte
  4. meiosis 2
  5. nuclei fuse –> zygote forms (~12 hours later)
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5
Q

cleavage division

A

mitotic division of the zygote

daughter cells called blastomers

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6
Q

2 systems for dating pregnancies

A
  • fertilization age

- menstrual age

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7
Q

fetilization age

A

dates pregnancy from the time of fertilization, thus a six week embryo is six weeks (42 days) from the day of fertilization

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8
Q

menstrual age

A

Used by clinicians and dates the pregnancy from the woman’s last normal menstrual period (menstrual age). (divided in trimesters) The menstrual age of a human embryo is two weeks greater than the fertilization age because usually two weeks elapse between the start of the last menstrual period and fertilization

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9
Q

embryonic stages of development

A
  • embryonic stage
  • fetal stage
  • postnatal
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10
Q

embryonic stage

A

first 8 weeks

  • zygote formation
  • cell division
  • implantation
  • organ formation
  • teratogen sensitivity
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11
Q

fetal stage

A

week 9 to birth

  • rapid fetal growth
  • sex organ formation
  • organ system function
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12
Q

postnatal

A

emotional, physical, and social growth

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13
Q

outer cell mass/ trophoblast

A

gives rise to the extra-embryonic tissues that form the placenta

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14
Q

inner cell mass/ embryoblast

A

gives rise to the embryonic cells and layers that form all of the tissues and organs of the body (intra-embryonic)

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15
Q

stages in human implantation

A

5-maturation of blastocyst
5-loss of zone pellucida
6-attachment of blastocyst to uterine epithelium
6-7- epithelial penetration
7-9-trophoblastic plate formation and invasion of uterine storm by blastocyst

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16
Q

what is the usual site of implantation

A

the posterior, superior wall of the uterus

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17
Q

ectopic pregnancy ?

A

“out of place”

happens ~ 0.25 to 1% of the time

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18
Q

where do most ectopic pregnancies occur?

A

ampulla of the uterine tube

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19
Q

rick factors for ectopic pregnancies

A

pelvic inflammatory disease, endometriosis

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20
Q

clinical signs for ectopic pregnancies

A

abnormal bleeding, abdominal pain, positive pregnancy test, intraperitoneal blood

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21
Q

decidua

A

name for endometrium after implantation

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22
Q

after implantation trophoblast proliferates into 2 layers

A

cytotropoblast layer

syncytiotropoblast

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23
Q

cytotropoblast layer

A

mitotically active inner layer of cells

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24
Q

syncytiotropoblast

A

produces human chorionic gonadotropin (hCG)- feedback to the ovary to the corpus luteum (hormones maintain the pregnancy until the placenta is fully functioning)

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25
Q

after implantation the cells of the embryoblast reorganize into 2 epithelial layers

A

epiblast layer

hypoblast layer

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26
Q

epiblast layer

A
  • form a membrane that lines the amniotic cavity
  • dorsal aspect of the disc
  • gives rise to the embryo
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27
Q

hypoblast layer

A
  • form a membrane that lines the yolk sac
  • also called the primitive endoderm
  • does NOT contribute to the cells developing embryo
  • ventral aspect of the disc
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28
Q

2 cavities that form

A
  • amniotic cavity

- yolk sac/ umbilical vesicle

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29
Q

amniotic cavity

A

• The amniotic membrane surrounds the embryo
following body folding
• Prevents mechanical injury to the fetus
• Allows normal fetal movements
• Cavity expands until fluid content reaches nearly one L by 33 weeks
• Sources of amniotic fluid:
o Amnion cells
o Maternal tissue (diffusion across chorionic plate)
o Fetal urine

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30
Q

extraembryonic mesoderm

A

forms between the yolk sac and cytotrophoblast

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31
Q

chorionic cavity divides the extraemrbyonic mesoderm into the

A
  • extraembryonic somatic mesoderm

- extraembryonic splanchnic mesoderm

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32
Q

extraembryonic somatic mesoderm

A

lining cytotrophoblast and amnion

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33
Q

extraembryonic splanchnic mesoderm

A

lines the yolk sac

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34
Q

the chorion forms the wall of the chorionic cavity and has 3 layers

A
  • extraembryonic somatic mesoderm
  • cytotrophoblast
  • syncytiotrophoblast
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35
Q

connecting stalk

A

suspends the amniotic cavity and the yolk sac in the chorionic cavity
-future umbilical cord

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36
Q

contact to the maternal blood

A

ALL urfaces of chorion and villi in contact with maternal blood are lined with syncytiotrophoblast**

37
Q

villi

A

numerous , minute, elongated projections

38
Q

primary villi

A

made up of syncytiotrophoblast and cytotrophoblast

39
Q

secondary villi

A

made up of syncytiotrophoblast, cytotrophoblast, mesoderm core

40
Q

tertiary villi

A

made up of syncytiotrophoblast, cytotrophoblast, mesoderm core, and villous capillary

41
Q

the placenta

A

is formed by both maternal and fetal contributions:
Fetal -the structures of the chorion
Maternal - the decidua

42
Q

Week two = “rule of two’s”:

A
  • Embryoblast splits into two layers (epiblast, hypoblast)
  • Trophoblast gives rise to two tissues (cytotrophoblast, syncytiotrophoblast)
  • Two yolk sacs form (primary, secondary)
  • Two new cavities form (amnionic, chorionic)
  • Extraembryonic mesoderm splits into two layers (somatic, splanchnic)
43
Q

week three

A

(15-16 days post fertilization) gastrulation occurs producing 3 main germ layers

44
Q

primitive streak

A

represents site of cell migration
CAUDAL to CRANIAL
disappears at the end of the 4 week

45
Q

primitive node

A

elevated area at the cephalic end of the primitive streak

46
Q

epiblast cells along the primitive streak

A

epiblast cells proliferate, lose their adhesion molecules(E-cadherin), detach from their neighboring cells and migrate toward the median plane of the embryonic disc

47
Q

epiblast layer gives rise to

A

all 3 germ layers

48
Q

endoderm

A

forms by epiblast cells that replace the entire hypoblast

49
Q

mesoderm forms..

A

by epiblast cells that migrate between the existing layers

50
Q

ectoderm forms..

A

by cells that remain in the epiblast after gastrulation is complete

51
Q

Sacrococcygeal teratoma

A

remnants of the primitive streak remain in the sacral region and cells proliferate and form a tumor
most common newborn tumor

52
Q

notochord

A

axial mesoderm that forms a long chord along the cranial- caudal axis

53
Q

Ectoderm and endoderm are adhered together at the

A

oropharyngeal and cloacal membranes

-but two membranes will break down completely as development continues

54
Q

prechordal plate

A
  • mesoderm structure

- may act as a signaling center to stimulate the development of the forebrain and contribute to head and neck mesenchyme

55
Q

importance of the notochord

A

-vertebral column and base of skull develop around it
-inductive signals (induction-when one population of cells influences the development of another population of cells) stimulate:
o the conversion of overlying surface ectoderm into neural tissue.
o the transformation of mesodermal cells of the somites into vertebral bodies.

the adult remnant: nucleus pulposus of the intervertebral disc.

56
Q

neural plate

A

thickened region composed of columnar epithelial cells–> neuroectoderm
develops into the primordial central nervous system

57
Q

bending of the neural plate

A
  • the edges thicken and move up to form the neural folds

- U shaped neural groove forms in the center

58
Q

closure of the neural tube

A
  • neural folds migrate to the midline and fuse
  • neural crest dissociates to become the neural crest cells
  • the remaining ectoderm differentiates into the epidermis layer (of skin)
59
Q

day 22

A

lateral edges of the neural folds first begin to fuse in the occipitocervical region

60
Q

day 24

A

cranial neuropore of the neural tube closes

61
Q

day 26

A

caudal neuropore of the neural tube closes

62
Q

Spina bifida with meningocele

A

-sac contains meninges and CSF, may be spinal anomalies if nerve roots extend into sac

63
Q

Spina bifida with meningomyelocele

A

-severe with neurological defects below the level of the lesion

64
Q

Spina bifida with myeloschisis (rachischisis)

A

-neurofolds fail to fuse

65
Q

Meroanencephaly

A
• Failure of the rostral neuropore to
close during the fourth week
• Forebrain development is abnormal
• Some, or all of the brain stem is intact
• Overlying bone is defective (calvaria)
66
Q

neural crest cells

A
  • neural crest cells give rise to the cells and tissues of the peripheral nervous system as well as many other critically important structures
  • derived from ectoderm related to the neural tube
  • often called fourth germ layer
  • must migrate from their site of origin undergo ectodermal to mesenchymal differentiation (migrate into the mesenchyme on each side of the tube)
  • very vulnerable cells
  • ectomesenchyme
67
Q

derivatives of surface ectoderm

A
  • epidermis
  • nails
  • hair
  • subcutaneous glands
  • mammary glands
  • anterior pituitary
  • enamel
  • lens of eye
68
Q

derivatives of neural ectoderm

A
  • central nervous system
  • retina
  • posterior pituitary gland
  • pineal body
69
Q

regions of mesoderm

A

paraxial, intermediate, lateral mesoderm

70
Q

Paraxial mesoderm

A

• Bilateral- next to (para) axial mesoderm
• Organizes into segments called somitomeres in
the head region
• From occipital region caudally somitomeres organize into somites
• The age of the embryo can be correlated to the number of somites

71
Q

each somite gives rise to…

A

its own sclerotome, myotome and dermatome

72
Q

sclerotome

A

segmental bone (axial skeleton)

73
Q

myotome

A

skeletal muscle

74
Q

dermatome

A

dermis/ connective tissue of the back

75
Q

each myotome and dermatome…

A

has its own segmental nerve component that migrates with the cells

76
Q

intermediate mesoderm

A

• Gives rise to the urogenital system
• Functionally divided into urinary
system and genital system

77
Q

lateral mesoderm

A

the lateral (plate) mesoderm will develop cavities that coalesce and separate the lateral mesoderm into the:

  • somatic (parietal) mesoderm layer
  • splanchnic (visceral) mesoderm
78
Q

The somatic mesoderm and overlying ectoderm will form

A

the body wall

79
Q

The splanchnic mesoderm and underlying endoderm form

A

the gut wall

80
Q

the body cavity is formed by…

A

splitting of the lateral plate mesoderm

81
Q

mesothelium

A

differentiated specialized simple squamous epithelium of cells lining the parietal and visceral layers
-lines the intraembryonic cavities

82
Q

The space between the two layers of lateral plate mesoderm is the primitive body cavity which gives rise to the:

A
  • Peritioneal cavity
  • Pleural cavities
  • Pericardial cavity
83
Q

Folding occurs in simultaneously in two planes:

A
  • longitudinal or median plane(cranial to caudal) occurs due to brain development
  • horizontal plane (lateral body fold) occurs due to growth of the somite
84
Q

cranial fold/ head fold

A

involves:
- septum transversum (the primordial diaphragm)
- primordial heart
- pericardial cavity
- oropharyngeal membrane

endoderm is incorporated into the embryo and forms the foregut

85
Q

tail fold

A

involves:
- primitive streak
- cloacal membrane
- connecting stalk

endoderm lining the yolk sac is incorporated as the hindgut

86
Q

lateral folding

A

growth of somites forms the body wall

endoderm is incorporated as the midgut

87
Q

endoderm gives rise to

A
  • epithelium of the gut tube
  • liver, gallbladder, pancreas
  • epithelium ofrespiratory system
  • epithelial lining of the urinary bladder and urethra
  • epithelium of some of the glands and structures of the head and neck
88
Q

Omphalocele

A

o Failure of the intestines to return to the body cavity
o Covered by amnion
o Often associated with other malformations

89
Q

Gastroschisis

A

o Protrusion of the viscera into the amniotic cavity due to abnormal closure of the body wall
o Viscera are not covered by amnion