Lecture 2 - CAMs and Junctional Complexes Flashcards
Which cell adhesion molecule is associated with occludens and desmosomes (macula adherens)?
Cadherins
Which molecular complex primarily serves to prevent paracellular transport?
Zonula occludens
Which molecular complex is ONLY associated with the basal domains?
Hemidesmosomes
Which CAMs are Calcium dependent?
Cadherins and Selectins
Which CAMs are Calcium independent?
Integrins and Immunuglobulin superfamily molecules
Which CAM recognizes carbohydrates?
Selectins
Desmocollins and desmogleins (located in intercellular spaces) belong to which CAM?
Cadherins
Which CAM recognizes extracellular matrix molecules, such as fibronectin and laminin, as a Ligand?
Integrins
Which CAM recognizes Integrins as a Ligand?
Ig Superfamily
Hemidesmosomes belong to which CAM?
Integrins
State where each of the following Cadherins are found:
E-cadherins, N-cadherins, P-cadherins
Epithelial tissues, nerve cells, placenta
Which protein serves as a major interface between the cadherins that hold adjacent cells together and their actin cytoskeletons?
Catenins
State where each of the following Selectins are found:
L-selectin, E-selectin, P-selectin
Lymphocytes, Endothelial cells, platelets.
Which molecular complex anchors cells together and reinforces the physical integrity of tissues and the cells that make up the tissues?
Zonula adherens
Talin, Vinculin, and alpha-actinin are used as connecting proteins with which CAM?
Integrins
What is a tonofilament? Give one example.
Proteins that generate or resist tension. Example: Keratin.
N-CAM, ICAM-1 and ICAM-2 are members of which CAM?
Immunoglobulin superfamily.
Which junctional complex anchor cells together and reinforce the physical integrity of tissues and cells that make up the tissues?
Adherens. “adhere” - hold cells together
Which junctional complex establishes an impermeable barrier between adjacent cells, especially epithelial cells, that prevents paracellular transport and maintains concentration differences between the environments?
Occludens. “occlude” - keep stuff out
Which junctions are composed of molecular pores that enable cells to rapidly exchange ions and small molecules that help coordinate activities among the cells?
Gap junctions.
Which junctional complex travels all the way around the circumference of the cell and link all neighboring cells to the central cell? (belt-like complexes)
Zonula, can be either adherins or occludens. Zonula associate with intracellular actin microfilaments.
Which junctional complex is a “spot” or snap-like junction and associate with intracellular intermediate filaments (tonofilaments)?
Macula. They are of the adherens variety. Referred to as desmosomes when associated with the lateral domains and hemidesmosomes when associated with the basal domain.
What is type of plaques are desmogleins and desmocollins anchored to?
Desmoplakin and Plakoglobin.
What proteins are associated with the junctional complex and are responsible for the occlusive properties of the tight junction?
Claudins and Occludins
What are Unique components of the junctions:
finish later
What are common components of the junctions:
finish later
How many connexins are used to form one connexon and which junctional complex are they apart of?
6, Gap junctions
