Lecture 2/14/25 Flashcards
DNA Replication
What is the role of DNA polymerase III?
It is the main replicative enzyme in bacteria, synthesizing most new DNA.
What happens when DNA polymerase III meets an RNA primer?
It halts, allowing for primer removal and gap-filling by another polymerase.
How is an RNA primer removed and replaced?
DNA polymerase I removes the primer and fills in the gap with DNA.
What are single-stranded binding (SSB) proteins?
They bind to single-stranded DNA to prevent secondary structure formation and degradation.
What is a holoenzyme?
A multi-subunit complex that carries out highly processive DNA replication.
What is the role of the sliding clamp?
It tethers DNA polymerase to DNA, increasing replication efficiency.
What is the bacterial sliding clamp called?
The B clamp.
What does the clamp loader do?
It assembles the sliding clamp onto DNA to facilitate polymerase binding.
