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Microbiology Lecture > Lecture 13: DNA Replication > Flashcards

Lecture 13: DNA Replication Flashcards

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1
Q

Binds to the origin and pulls strands apart

A

origin binding protein

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2
Q

Moves down the template 5’-3’ direction and uses ATP to break H bonds

A

Helicase

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3
Q

Makes the RNA primer (makes phosphodiester bonds)

A

Primase

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4
Q

keeps the template strands apart

A

single strand binding protein

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5
Q

extends RNA primer

A

DNA Pol III

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6
Q

removes and replaces RNA primer

A

DNA Pol I

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7
Q

Connects Okazaki fragments

A

ligase

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8
Q

What are the steps of DNA replication?

A
  1. Yank DNA apart and lay down RNA primer
  2. Add DNA nucleotides by DNA Pol III
  3. Remove RNA primer
  4. Ligate Okazaki fragments
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9
Q

Bi-directional DNA replication with 2 replication forks

A

Replication on circular prokaryotic chromosomes

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10
Q

In replication, in circular prokaryotes, the _______ strand is going toward the origin

A

lagging

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11
Q

In replication, in circular prokaryotes, the _______ strand is going away from the origin

A

leading

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12
Q

What direction does helicase move on the template or parental strand?

A

5’-3’ direction

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13
Q

What is the energy source it uses to break H bonds?

A

ATP

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14
Q

What bond does SSB keep from reforming?

A

H Bonds

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15
Q

Where does helicase get its energy to perform its main function?

A

ATP

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16
Q

What were the nucleotides before they were added to the new strand?

A

Nucleoside Triphosphate

17
Q

Why do Nucleotides start as Nucleoside Triphosphates?

A

Provide energy by breaking off pyrophosphate and then broken down

18
Q

______ is the RNA Pol involved in ___ _________ whereas ____ __________ is involved in transcription.

A

Primase; DNA Replication; RNA Polymerase

19
Q

DNA replication is semiconservative; this should demonstrate why ____ ____ ____ is the enzyme that extends the RNA primer once it has been completed.

A

DNA Pol III

20
Q

Adding DNA nucleotides by DNA Pol III

A

DNA replication

21
Q

The ribonucleotide at the 5’ end of the RNA primer on the lagging strand is removed using the ____ __________ activity of ___ ____ _

A

5’-3’ exonuclease; DNA Pol I

22
Q

DNA replication

A

removal of RNA primer

23
Q

DNA Pol I continues to remove RNA primer using ____ exonuclease activity and fills with DNA

A

5’-3’

24
Q

The bond is made by DNA ligase and requires ____ breakdown to drive the reaction

A

NAD+

Microbiology Lecture (6 decks)

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