LECTURE Flashcards
A 52-year-old woman with a medical history of complicated urinary tract infection
readmitted due to high fever, severe chest pain, fatigue, aching joints and muscles and
difficult urination. Colorless colonies were isolated on MacConkey agar, positive on indole,
urease, citrate and malonate test. What is the possible microorganism?
A. Serratia marcescens
B. Citrobacter diversus
C. Escherichia coli
D. Proteus vulgaris
Proteus vulgaris
Which of the following is/are best to cultivate the Nocardia species?
I. 5-7 days of incubation at 37C
II. Incubate at room temperature
III. 3-6 days of incubation at 37C
IV. Use BCYE and Chocolate Agar Plate
III and IV
The following results are obtained from a pure culture of gram negative rods recovered
from the pulmonary secretions of a 10-year-old cystic fibrosis patient with pneumonia
Oxidase =blue Motility = with turbidity at SIM
Glucose OF (open) = yellow Gelatin =liquefied
Pigment = green Arginine dihydrolase =purple
Growth at 42’C = positive Flagella = polar monotrichous
Which is the most likely organism?
A. Pseudomonas stutzeri
B. Pseudomonas pseudomallei
C. Pseudomonas aeruginosa
D. Pseudomonas cepacia
Pseudomonas aeruginosa
High fever and an inflammatory swelling of the axilla and groin lymph nodes are signs and
symptoms of 35-year-old female patient admitted at Emergency room. Four blood cultures were obtained, resulting in
growth of gram-negative bacilli resembling safety pins and showing colorless colonies on MacConkey Agar. Testing demonstrated a non-motile bacilli that was biochemically inert. What is the most likely identification?
A. Klebsiella oxytoca
B. Yersinia enterocolitica
C. Morganella morganii
D. Yersinia pestis
Yersinia pestis
An isolate of Escherichia coli recovered from the stool of a patient with severe bloody
diarrhea should be tested for which carbohydrate before sending it to reference laboratory
for serological testing?
A. Raffinose
B. Mannitol
C. Sucrose
D. Sorbitol
Sorbitol
True about the cell wall of partially acid-fast bacilli, except:
I. contain shorter mycolic acid
II. peptidoglycan made up of mesodiaminopimelic acid
III. contain glucose and arabinose
IV. forms the filaments
III and IV
TRUE OR FALSE
Morse code appearance is seen in gram staining of Aggregatibacter actinomycetemcomitans
False
A curved gram-negative rod producing oxidase-positive colonies on blood agar
was recovered from a stool culture. Given the following results, what is the most
likely identification?
Lysine decarboxylase = positive Arginine decarboxylase = Negative
Indole = positive TSI = Alk/Acid
VP = Negative Lactose = Negative
Urease = variable String test = Negative
TCBS agar = Green colonies
A. Vibrio cholerae
B. Salmonella spp
C. Shigella spp
D. Vibrio parahaemolyticus
Vibrio parahaemolyticus
A 28-year-old female complained of a severe eye irritation after removing her softcontact lenses. Swab from the infected right eye was submitted in the microbiology
for culture and sensitivity. At 24 hours, growth of a gram-negative rod that tested
positive for cytochrome oxidase was noted. Bluish-green colonies were showed on
Mueller-Hinton Agar. What is the most likely microorganism?
A. Stenotrophomonas maltophilia
B. Burkholderia cepacia
C. Pseudomonas aeruginosa
D. Acinetobacter baumanii
Pseudomonas aeruginosa
A catheterized urine specimen collected from a female patient who is residing in a nursing
home reveals the following:
TSI: K/A, no gas, H2S negative MacConkey: clear colonies
indole: red ring Citrate: blue
Motility: positive Urease: pink
Deaminase: burgundy
Identify the isolate:
A. Providencia stuartii
B. Klebsiella pneumoniae
C. Escherichia coli
D. Proteus mirabilis
E. Serratia marcescens
Providencia stuartii
An acid fast organism is isolated form a wound culture taken from the hand of a marine
biology student The organism produces smooth, bright lemon-yellow colonies on
Lowenstein-Jensen agar when exposed to light The organism is negative for nitrate
reduction and is catalase and urease positive. The identity of this organism is:
A. Mycobacterium scrofulaceum
B. Mycobacterium tuberculosis
C. Mycobacterium fortuitum
D. Mycobacterium marinum
E. Mycobacterium ulcerans
Mycobacterium marinum
Which of the following tests best differentiate Shigella species from E. coli?
A. Hydrogen sulfide, VP, citrate, and urea
B. Hydrogen sulfide, ME, citrate, and urea
C. Gas, citrate, and VP
D. Lactose, indole. ONPG; and motility
Lactose, indole. ONPG; and motility
Formation of granuloma into the lungs that may extend into the tissue producing extensive
tissue involvement and damage.
A. Confluent bronchopneumonia
B. Pulmonary tuberculosis
C. Chronic Obstructive Pulmonary Disease
D. Lobar pneumonia
Confluent bronchopneumonia
Which of the following Mycobacterium species is diagnosed by biopsy other than culture?
A. M. bovis
B. M. leprae
C. M. avium
D. M. canetti
M. leprae
Which are the best two tests to differentiate Aeromonas hydrophilia from Plesiomonas
shigelloides?
A. DNase and VP
B. Growth on MacConkey and blood agar
C. Oxidase and motility
D. Indole and lysine decarboxylase
Growth on MacConkey and blood agar
1 Statement: All Non-fermentative Organisms are oxidase positive
2 Statement: Acinetobacter species is the negative in motility and oxidase.
A. Neither Statements are correct
B. 1 Statement is true, 2 Statement is true
C. 1 Statement is false, 2 Statement is true
D. 2 Statement is false, 1 Statement is true
1 Statement is false, 2 Statement is true
A bloody stool cultured from a 35-year-old- woman after three days of severe diarrhea
showed the following results at 48 hours after being plated on the following media:
Mac Conkey agar: colorless colonies
Hektoen Enteric Agar: Blue-green colonies
Oxidase: colorless
Indole: colorless ring
SIM: No brush appearance from the stab line without H2S
Urease: Orange
ONPG: yellow
A. Escherichia coli
B. Salmonella cholerae- suis
C. Salmonella typhi
D. Shigella sonnei
Shigella sonnei
3 most commonly isolated Non-fermentative Organism
A. Burkholderia pseudomallei
B. Pseudomonas aeruginosa
C. Burkholderia cepacia
D. Stenotrophomonas maltophilia
Stenotrophomonas maltophilia
The Shiga-like toxin is produced mainly by which Enterobacteriaceae?
I. Shigella dysenteriae
II. Enteropathogenic Escherichia coli
III. Enterohemorrhagic Escherichia coli
IV. Enteroadherent Escherichia coli
Enterohemorrhagic Escherichia coli
A wound sample was submitted at Microbiology reveals non-motile gram-variable bacilli,
aerobic and catalase positive. Identify the isolate:
A. Streptomyces anulatus
B. Gordonia bronchialis
C. Actinomadura madurae
D. Rhodococcus equi
Gordonia bronchialis
A gram-negative coccobacillus was isolated from an infected cat bite. The isolate grew on
blood agar with a musty smell, but did not grow on MacConkey agar. It was catalase,
indole, and oxidase positive and negative to urease. Which one of the following is the most
likely identity of this isolate?
A. Pasteurella multocida
B. Eikenella corrodens
C. Pasteurella canis
D. Pasteurella bettyae
Pasteurella multocida
Xylose lysine deoxycholate (XLD) agar is a highly selective medium used for the recovery
of which bacteria?
A. Yersinia species that do not grow on Hektoen Enteric Agar
B. Enterobacteriaceae from gastrointestinal specimens
C. Streptococcus species from stool cultures
D. Corynebacterium species from normal flora
Enterobacteriaceae from gastrointestinal specimens
TRUE OR FALSE
Rosette form is the microscopic morphology of Cardiobacterium hominis
True
Grown inhibition by thiophene-2-carboxylic hydrazide (T2H) is used to differentiate
Mycobacterium tuberculosis from which otter Mycobacterium sp.?
A. M. avium complex
B. M. bovis
C. M. marinum
D. M. kansasii
M. bovis
1 Statement: All Partially acid-fast bacilli are strictly aerobic
2 Statement: All Non-Partially Acid-fast Bacilli are lysozyme resistant.
A. Neither Statements are correct
B. 1 Statement is true, 2 Statement is true
C. 1 Statement is false, 2 Statement is true
D. 2 Statement is false, 1 Statement is true
1 Statement is false, 2 Statement is true
Which test group best differentiates Acinetobacter species. from Pseudomonas.
aeruginosa?
A. Oxidase, motility, 42°C growth
B. MacConkey growth, 37C growth, catalase
C. Blood agar growth, oxidase, catalase
D. Oxidase, TSI, MacConkeY growth
Oxidase, motility, 42°C growth
Direct spread of pneumonic plaque disease occurs by which route?
Airborne droplets
All of the isolates of Eikenella corrodens pits the agar with bleach-like odor. True or false
false
A suspected case of plague requires which of the following procedures in order to confirm
Yersinia pestis?
I. Satellitism pattern in broth culture
II. Incubation of blood cultures at both 25°C and 30°C
III. Culture aspirates from buboes to MacConkey agar at room temperature
IV. safety pin appearance in leifson’s staining
A. II only
B. II, III
C. I, IV
D. II, III, IV
II only
Which of the biochemical tests best to differentiate Klebsiella pneumoniae from Klebsiella
oxytoca?
A. Citrate
B. Indole
C. Urease
D. Sucrose
Indole
A gram negative, motile rod is isolated from a urine culture. On trypticase soy agar, the
organism produces red pigmented, DNAse positive colonies. Additional biochemical
testing revealed the following: KCN positive, ODC positive, ADC negative and LDC positive.
The most probable organism is:
A. Escherichia coli
B. Proteus vulgaris
C. Pseudomonas aeruginosa
D. Enterobacter cloacae
E. Serratia marcescens
Serratia marcescens
Which of the follow is noted for a positive result for niacin accumulation?
A. Mycobacterium gordonae
B. Mycobacterium fortuitum-chelonae
C. Mycobacterium tuberculosis
D. Mycobacterium avium-intracellulare
Mycobacterium tuberculosis
Common name of Mycobacterium gordonae
tap water bacillus
Common name of Mycobacterium kansasii
yellow bacillus
Common name of Mycobacterium intacellulare
battey bacillus
Sputum samples for Tuberculosis are optimally collected
A. by collecting sputum for a 24 hour period for 1 day
B. in the morning on 3 consecutive days
C.in the evening on 3 consecutive days
D. by collecting sputum for three 24 hour periods
in the morning on 3 consecutive days
Colorless colonies on MacConkey Agar and yellow colonies on TCBS agar are
presumptive identification of Vibrio cholerae. Which biochemical tests would be
used to eliminate Aeromonas and Plesiomonas species in seafood food
poisoning?
I. Mannitol fermentation
II. String test
III. Gelatin Liquefaction
IV. Oxidase
A. II, IV
B. I, II
C. I, II, III
D. III, IV
I, II
Which of the following tests may be used to differentiate Cardiobacterium hominis from
Actinobacillus spp.?
A. Oxidase
B. Gram stain
C. Indole
D. Anaerobic incubation
Indole
Which of the following is the best media for the cultivation of Vibrio species?
A. TCBS, BAP, Alkaline Peptone Water
B. TCBS, BCYE, MacConkey, SSA
C. TCBS, Clary-Blair medium, SSA
D. TCBS, Alkaline Peptone Water, MacConkey
TCBS, Alkaline Peptone Water, MacConkey
Cetrimide agar is used as a selective isolation agar for which organism?
A. Burkholderia cepacia
B. Stenotrophomonas maltophilia
C. Acinetobacter baumannii
D. Pseudomonas aeruginosa
Pseudomonas aeruginosa