Lecture 12: Germ Layer Formation

Question 1

Expression of what molecule causes cells entering the ICM to become epiblast cells?

Answer 1

Nanog

Question 2

Expression of what molecule causes cells entering the ICM to become hypoblast cells?

Answer 2

Gata6

Question 3

What molecule, secreted by Nanog, may induce Gata6?

Answer 3

FGF-4

Question 4

What role does Wnt, TGF-B play in the formation of the primitive streak?

Answer 4

Induce formation of the primitive streak

Question 5

What are the 3 classical molecular markers expressed by nodal cells?

Answer 5

Chordin, Goosecoid, Foxa-2

Question 6

What are “bottle cells” and how are they related to gastrulation?

Answer 6

Bottle cells are named for the shape they assume when transforming from epithelium to mesenchymal cells - transformation may be stimulated by Snail

Question 7

What adhesion molecules are expressed in epithelium prior to induction?

Answer 7

E-cadherins and N-CAMs

Question 8

What adhesion molecules are expressed in overlying ectoderm after neural tube induction?

Answer 8

E-cadherins

Question 9

What adhesion molecules are expressed in neural tube ectoderm after induction?

Answer 9

N-CAMs, N-cadherins