Lecture 12 Flashcards
1
Q
what is not present in sera which is found in plasma?
A
clotting factors
2
Q
how is it possible that two different antibodies can bind the same antigen?
A
they recognise different epitopes
3
Q
what is a B cell hybridoma?
A
fusion between an immortal B cell tumour and a B cell
4
Q
why are secondary antibodies commonly used when antibodies are used for detecting the presence of an antigen?
A
secondary antibodies detect the primary antibody to its antigen, this increases its sensitivity by increasing the signal
5
Q
why is an indirect ELISA capable of detecting less antigen in a biological sample than a direct ELISA?
A
as it involves a secondary antibody and so increases the signal
