Lecture 1: Introduction- Cells and Microscopy Flashcards
What is Biology?
Biology is the study of life
What is an Organism?
An assembly of molecules functioning as a stable whole that exhibits life.
-Unicellular organisms
-Multicellular organisms
Cell theory:
- All organisms are composed of one or more cells
- The cell is the basic structural and functional unit of all living organisms.
- Cells arise only from the division of preexisting cells.
Basic Features of Cell Structure and Function
- Cells carry out the essential processes of life
- Cells have a DNA- containing central region
- Cells contain DNA and RNA
- Cells use energy
- Cells occur in prokaryotic and eukaryotic forms
Difference between Prokaryotic and Eukaryotic Cells
Prokaryotic cells lack a nucleus and have no membrane-bound organelles, while Eukaryotic cells do.
Magnification
The ratio of the object as viewed to its real size (e.g. 1200x)
Resolution
The minimum distance that 2 points in the specimen can be separated and still be seen as 2 points
Light Microscopes
Use light to illuminate the specimen
Electron Microscope
Use electrons to illuminate the specimen
Light Microscopy
- Bright field microscopy
- Differential interference contrast microscopy (DIC)
- Fluoresence microscopy
- Confocal microscopy
Electron Microscopy
- Transmission electron microscopy (TEM)
- Scanning electron microscopy (SEM)