Lecture 1. Food Microbiology and Food Related Pathogens Flashcards

1
Q

What are the three types of food borne disease?

A

Infection (bacterial, fungal, eukaryotic parasites, viruses)
Intoxication (bacterial and fungal toxins, shellfish toxins, metals, chemicals)
Allergy (usually specific proteins)

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2
Q

What is the causative chain of infection?

A

Agent → Source → Contamination → Suitability → Growth conditions → Consumption

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3
Q

What can food contamination be?

A

Biological, physical, chemical or allergen

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4
Q

What are the four sources of food contamination?

A

Food handler associated
Food processing
Food storage
Food product

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5
Q

How can food handler’s be a source of contamination?

A

Inadequate personal hygiene, faecal contamination

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6
Q

How can food processing be a source of contamination?

A

Equipment, packaging, personnel contamination

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7
Q

How can food storage be a source of contamination?

A

Time and temperature

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8
Q

How can food product be a source of contamination?

A

Meat and poultry – Contaminated feed, animal-animal transmission
Surface contamination (fur/feathers/hooves)
Fish and Shellfish - contamination sewage/water
Produce contaminated - contaminated irrigation water
Soil contamination

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9
Q

Compromised Seafood

A

Accumulate high levels of water associated bacteria (Crusataceans/molluscs)
Consumption of contaminated organisms (Crustaceans/fish)
Surface contamination from water related pathogens (Fish)
Susceptible to temperature abuse (retailer and customer)

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10
Q

Compromised meat and poultry

A

High levels of bacteria naturally found within organism (Poultry)
Cross contamination during processing (ready-to-eat meat and poultry)
High count of contact points in processed meats
Non-thermally treated products (Sausages)

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11
Q

Benefits of using microbes with food

A

Food enhancement (cheese, meat, wine and beer)
Food additives (food safety and stability, probiotics)

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12
Q

Drawbacks of using microbes with food

A

Food spoilage (shortened shelf life, failure to meet legal requirements)
Food safety (morbidity, mortalitly)

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13
Q

What is the food factor of Lactobacillus acidophilus?

A

Pro-biotic, yogurts, kefir, miso, sauerkraut, cheese

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14
Q

What are the four intrinsic treatments that preserve food?

A

Treatment: water activity, Action: growth inhibition
Treatment: pH - food acidity, Action: growth inhibition
Treatment: antimicrobial chemicals, Action: various
Treatment: biological (competitive mircoflora, bacteriocins, enzymes), Action: vaious

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15
Q

What are the four extrinsic treatments that preserve food?

A

Treatment: heat treatment, Action: destruction of cells and spores
Treatment: low temperature storage, Action: growth inhibition
Treatment: modified atmosphere packaging, Action: growth inhibition/destruction of cells
Treatment: radiation (UV, microwave, gamma), Action: destruction of bacterial cells

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16
Q

What is hurdle technology?

A

A number of preservation factors that are combined to improve food safety

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17
Q

What does hurdle technology allow?

A

Individual measures to be milder; increasing ‘naturalness’ of food

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18
Q

Is hurdle technology more effective than individual processing methods?

A

Yes

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19
Q

How many hospitalisations occur annually in the UK due to food-borne diseases?

A

~20,000

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20
Q

How many deaths occur annually in the UK due to food-borne diseases?

A

~500

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21
Q

What are the four main food-related pathogens?

A

Salmonella sp.
Campylobacter jejuni
Listeria monocytogenes
Escherichia coli

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22
Q

Where is salmonella found?

A

In the intestinal tract of birds and other animals

23
Q

How does human contamination occur due to salmonella?

A

Through consumption of contaminated animals, animal products or water

24
Q

What does salmonella result in?

A

Gastroenteritis or typhoid fever depending upon the type of Salmonella encountered

25
How many cases of salmonella occur each year in the UK?
~8500 although this is thought to be an underestimate
26
What are the symptoms of gastroenteritis?
Occasional vomiting Severe diarrhoea Mild fever Muscle aches Rarely fatal
27
What is the incubation period for gastroenteritis?
8-48 hours
28
What is the morbidity period for gastroenteritis?
3-6 days
29
What is the infection type of gastroenteritis?
Superficial infection of the gut
30
What are the symptoms of typhoid fever?
Occasional vomiting Occasional diarrhoea Severe fever Sore throat/Cough Occasionally fatal
31
What is the incubation period for typhoid fever?
10-14 days
32
What is the morbidity period for typhoid fever?
2+ weeks
33
What is the infection type of typhoid fever?
Invasive infection of the lymph nodes, liver, spleen and gall bladder
34
Where is campylobacter found?
Commonly inhabits the GI tract of animals Commonly found in poultry, red meat, untreated water
35
What does campylobacter consist of?
Non-pathogenic and pathogenic species, C. jejuni being the primary human pathogen within the genus
36
How can campylobacter be prevented?
Infection can be prevented by sufficient cooking, good hygiene and correct processing by food/water providers
37
What is listeria and where is it found?
Gram positive, rod shaped bacteria which is found commonly in the environment due to its resilient nature
38
How many species does the genus Listeria contain?
7 species, only one of which is a significant human pathogen (L. monocytogenes)
39
What is the incidence rate and mortality rate of L. monocytogenes?
Low incidence rate (<200 cases per year in the UK) Extremely high mortality rate (>25%)
40
What can listeria result in?
Mild non-invasive infection or potentially life threatening invasive syndromes (septicaemia and infection of the CNS)
41
What is the site of infection of non-invasive listeriosis?
Digestive system
42
What are the symptoms of non-invasive listeriosis?
Fever Muscle pains Nausea/vomiting Diarrhoea Chills
43
Who is at risk of non-invasive listeriosis?
No predisposing factors required
44
What is the site of infection of invasive listeriosis?
Blood CNS Brain
45
What are the symptoms of invasive listeriosis?
Severe headache Stiff neck Confusion Seizures Tremors
46
Who is at risk of invasive listeriosis?
Elderly Pregnant Young/neonate Immuno-compromised
47
What is the site of infection of enterohemorrhagic Escherichia coli?
Colon
48
What are the symptoms of enterohemorrhagic Escherichia coli?
Severe stomach cramps Vomiting Diarrhoea (blood?) Possible fever
49
Who is at risk of getting enterohemorrhagic Escherichia coli?
Elderly Young Newborns
50
What are the possible complications due to enterohemorrhagic Escherichia coli?
Development of Haemolytic Uremic Syndrome (HUS), infection in the kidneys
51
What is the site of infection of enterotoxigenic Escherichia coli?
Gut
52
What are the symptoms of enterotoxigenic Escherichia coli?
Diarrhoea Occasional vomiting
53
Who is at risk of getting enterotoxigenic Escherichia coli?
Elderly Young Newborns
54
What are the possible complications due to enterotoxigenic Escherichia coli?
Rare Usually self limiting (1-3 days), less deadly