Lecture 1 - Cell membranes (Drawing portion)

Question 1

Draw the basic structure of a phospholipid clearly outlining all the groups of the polar head and a kink in one of the fatty acid chains.

Answer 1

refer to notes

Question 2

Draw the PI 3 kinase phosphorylation of IP2 to generate PIP3

Answer 2

refer to notes

Question 3

Draw the phospholipase C cleavage of PIP3 to generate IP3 and DAG.

Answer 3

refer to notes

Question 4

Show the general activity of acetone (CH3C0CH3) (hydrophilic) in water.

Answer 4

refer to notes

Question 5

Show the general activity of 2-methyl propane (hydrophobic) in water.

Answer 5

refer to notes.

Question 6

Draw the general shape of a phospholipid packing into a lipid bilayer.

Answer 6

refer to notes

Question 7

Draw the general shape of a lipid molecule packing into a lipid micelle.

Answer 7

refer to notes

Question 8

Show the energetically unfavourable and favourable states of a bilayer.

Answer 8

Planar = unfavourable
Spherical = favourable

Question 9

Show the movement of hydrophobic molecules, small uncharged polar molecules, large uncharged polar molecules and ions across a synthetic bilayer.

Answer 9

Refer to notes.

Question 10

Show the general shape and location of each of the 7/8 membrane protein arrangements.

Answer 10

1. Single pass transmembrane (with lipid anchor)
2. Multipass transmembrane
3. Beta barrel
4. Non spanning integral membrane protein
5. Lipid conjugated
6. GPI anchor
   7/8. Peripheral membrane proteins (ECF and ICF side)

Question 11

Draw the experiments to identify lac mutants.

Answer 11

1. Irradiate cells (make mutants)
2. Plate onto full media
3. Replicate onto media with lactose but no other sugar