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Advanced Cell Biology > final exam study guiderino > Flashcards

final exam study guiderino Flashcards

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1
Q

GHRH pathway

A

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2
Q

olfactory system GPCR pathway

A

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3
Q

PLC/Ca2+ signaling

A

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4
Q

photoreceptors and GPCRs

A

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5
Q

GPCR desensitization

A

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6
Q

Signaling via membrane soluble molecules and intracellular receptors

A

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7
Q

Auxin pathway

A

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8
Q

MT dynamic instability in vivo

A

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9
Q

Nucleation of MTs

A

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10
Q

censtronsomes and centrioles replication unto primary cilium

A

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11
Q

axonemal dynein movement

A

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12
Q

kartageners syndrome and nodal pathway

A

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13
Q

identification of cytoplasmic motor protein, house keeping information.

A

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14
Q

in vitro synthesis of MTs for identification of cytoplasmic motor protein

A

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15
Q

SDS-page experiment thing for id of motor protein

A

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16
Q

in vitro assay for motor activity

A

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17
Q

identification of a cytoplasmic motor protein

A

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18
Q

kinesin movement on MTs

A

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19
Q

Dynein motor activity

A

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20
Q

Treadmilling

A

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21
Q

Different actin regulatory proteins and functions

A

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22
Q

Regulation of myosin II by MLCK

A

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23
Q

Organization of a sarcomere

A

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24
Q

Muscle contraction

A

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25
Q

Major motor proteins: direction, tightly boound to cytoskeleton compionent when, processivity type

A

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26
Q

Ash1 mRNA localization in budding yeast

A

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27
Q

How was it shown that MTs and actin are necessary for mRNA localization in the Drosophila oocyte?

A

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28
Q

Fusion of head domains of MT motors to B-gal reveal MT orientation

A

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29
Q

Bcd and osk mRNA transport by kinesin and dynein. (how was it shown)

A

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LOF mutants accumulate wehre

30
Q

Egl interacts with DLC

A

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ip and western

31
Q

BicD and Egl proteins interact.

A

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GST pulldown

32
Q

Egl binds to 3’ UTR sequences

A

ITS and ILS = hint

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33
Q

Yeast 2-hybrid interaction assay

A

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34
Q

BicD and others hybrid asasy

A

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35
Q

mRNA transport in Drosophila oocytes

A

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Advanced Cell Biology (15 decks)

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