Lecture 1 Flashcards
Microorganisms
Found everywhere
Some can cause disease
Lack complexity
Prokaryotic
cells lack a true membrane-delimited nucleus
Eukaryotic
membrane-enclosed nucleus
complex
larger
Bacteria
Single celled
peptidoglycan wall
membrane bound nucleus
can live in extreme environments
Archaea
unique ribosomal RNA (rRNA) gene sequences
Lack peptidoglycan in cell walls
unique membrane lipids
extreme environments
Eukarya
Comprised of Protists and fungi
Protists
Generally larger than Bacteria and Archaea
Algae – Photosynthetic
Protozoa – May be motile, “hunters, grazers”
Slime molds – Two life cycle stages
Water molds – Devastating disease in plants
Fungi
Yeast – Unicellular
Mold – Multicellular
Acellular Infectious Agents
Prions
Viroids and virusoids
Viruses
Viruses
Smallest of all microbes
Requires host cell to replicate
Cause range of diseases, some cancers
Viroids and virusoids
Infectious agents composed of RNA
Prions
Infectious proteins
Origins of Life
Swartkoppie chert
Fossil record sparse
Swartkoppie chert
Granular silica deposits
Earliest Molecules - RNA
Original molecule must have fulfilled protein and hereditary function
Ribozymes
Ribozymes
RNA molecules that perform cellular work
16S rRNA
1,500 nucleotide RNA molecule
Hyper variable regions
Different in every species (16S Ribosomal RNA)
Highly conserved regions
Identical in all species (16S Ribosomal rRNA)
Last Universal Common Ancestor (LUCA)
Archaea and Eukarya evolved independently of Bacteria
Archaea and Eukarya diverged from common ancestry
evolution
leads to diversity
Mutation
genetic material led to selected traits
Discovery of Microorganisms
Antony van Leeuwenhoek
Spontaneous generation
Idea that living organisms (microbes) can develop from nonliving or decomposing matter
Miasma theory
Disease caused by foul air and bad smells
Robert Koch
Established the relationship between Bacillus anthracis and anthrax
These criteria now known as Koch’s postulates
Taxonomy
Classification
Nomenclature
Identification
Classification
arrangement of organisms into groups (taxa; s., taxon)
Nomenclature
assignment of names to taxa
Identification
determination of taxon to which an isolate belongs
Taxonomic Ranks - 1
Highest rank is domain
Bacteria and Archaea – microbes only
Eukarya – microbes and macroorganisms
Within domain
Phylum, class, order, family, genus, species (some microbes have subspecies)
Species
population of cells with similar characteristics”
Strain
group of cells derived from a single cell”