Lect 23-24 Molecular Diagnostics

Question 1

PCR

Answer 1

STR’s (Short tandem repeats) specific to each person. Can use them to see who parents are based on CPR/gel plating similarities

Question 2

Microarrays

Answer 2

See how much a gene is expressed (high green = high expression)

Uses reverse transcriptase

Question 3

SDS-Page

Answer 3

Separates proteins based on charge using pH

Question 4

Agarose gel

Answer 4

separates any molecule (e.g. DNA) based on charge (smaller DNA strand migrate further)

Question 5

The ___ domain on an antibody contains the antigen binding site

Answer 5

Fab

Question 6

Epitope

Answer 6

specific site on antigenic molecule recognized by antibody receptor

Question 7

ELISA - AB test to show if Ag is present

____ ELISA is used for ____ and should be backed up with a ______ ____

___ ELISA is used for ____

Answer 7

Indirect ELISA = HIV tests (tests person’s antibodies) and should be confirmed with Western blot

Sandwich ELISA = prego tests (tests for Ag)