[Lec 9] Key Terms: Splicing

Question 1

introns

Answer 1

intervening sequences in the coding DNA that has been removed

Question 2

exons

Answer 2

sequences that remain in the mRNA

Question 3

alternative splicing

Answer 3

different proteins encoded by same gene

Question 4

snRNPs

Answer 4

recognize the 5’ splice site and branch site; brings branch site and 5’ splice site together; catalyzes the RNA to cleave and join reactions

Question 5

U2AF

Answer 5

made up of 2 subunits; binds to py tract and smaller subunit binds to 3’ splice site; larger interacts with BBP and helps protein bind to branch site (early branch site)

Question 6

U2

Answer 6

binds to branch site, aided by U2AF and displaces BBP; known as A complex

Question 7

U4, U5, and U6

Answer 7

tri-snRNP particles that makes the B complex

Question 8

Exon Shuffling

Answer 8

overlaps with functional domains of protein (individually folding domains)