lec 9

Question 1

what is the purpose of kreb’s cycle?

Answer 1

produce:
- 6 NADH
- 2 ATP
- 2 FADH2

Question 2

what is the purpose of glycolysis?

Answer 2

• turn 6C glucose into 2 x 3C pyruvates (via aldolase)
• produce 2 net ATP (2 used, 4 gained)
• produce 2 NADH2

Question 3

how the FUCK does the ETC chain work

Answer 3

buckle in pussies we’re about to go turbo

1. NADH –> 2e- + (H+) + NAD+
   electrons are handed off to c1
2. c1 doesn’t want e-, so it gives e- to Q
3. at the same time, at c2, succinate donates e- to FAD+ to become FADH2
   c2 doesn’t want e-, so it also gives e- to Q
4. Q donates 2 e- to c3
5. c3 doesn’t want e- –> gives to cyt C
6. cyt C donates e- to c4, which gives it to oxygen (forms water)
7. hella H+ buildup in the intermembrane space. ATP synthase provides a channel for H+ to move down the gradient back into the matrix, at the same time producing ATP

Question 4

is glycolysis aerobic? what about kreb’s cycle? ETC?

Answer 4

glycolysis and kreb’s do not require oxygen. ETC does.

Question 5

what is the purpose of converting pyruvic acid to lactic acid if we have the ETC?

Answer 5

LDH pathway doesn’t require oxygen + can reliably regenerate NAD to repeatedly get energy

drawback is that lactic acid is produced as a byproduct and that’s not good for the body :(

Question 6

what are phosphogens? what are they good for? how does the mice experiment exhibit this?

Answer 6

high energy storages similar to ATP where it has a Pi chilling ready for use

creatine phosphate + ADP <–> creatine + ATP

good for burst activity! (mice experiment where creatine gene was knocked out)

Question 7

compare aerobic, anaerobic, and phosphagen use in terms of:
- stability
- energy yield
- recovery

Answer 7

stability: aerobic > anaerobic, phosphagen

energy yield: aerobic > anaerobic > phosphagen

recovery (to aerobic): phosphagen > anaerobic (anaerobic requires addressing all the lactic acid buildup)