Lec 7: Immunochemistry & EM Flashcards
It includes in situ detection of antigens in tissue sections and cells using monoclonal & polyclonal antibodies
Immunohistochemistry
Protocol for IHC staining (10)
- Deparaffinization & rehydration
- antigen retrieval
- peroxide block
- power block
- primary antibody
- super enhancer
- secondary antibody
- chromogen
- counter stain
- aqueous mount
What are barrier slides?
Positively charged slides with barriers to prevent loss of reagent
Dewaxing & rehydration
- This process is free from what chemical?
- How many changes in 3 minutes?
- Xylene and its substitutes
- 3 changes
Antigen Retrieval
- How many slides in 1 run?
- (time) at (temperature) in 2 cycles.
- 96 slides
- 16 minutes at 95°C in 2 cycles
The protocols for IHC staining which uses Phosphate buffered saline in rinsing before the next step
- antigen Retrieval
- peroxide block
- primary antibody
- super enhancer
- secondary antibody
- chromogen
Peroxide block & Power block
- (time) at (temperature)
- 10 minutes at RT (20°–25°C)
The protocols that use distilled water in rinsing before the next step
Counter stain
It is the microscopic study of tissues with the aid of antibodies that bind to tissue components and reveal their presence
Immunochemistry/immunlhistology
It involves antibodies to antigens which are attached to a color reagent via a series of stages
Immunochemistry/immunlhistology
It is produced by plasma cells and lymphocytes for protection against antigens (bacteria, virus, toxins)
Immunoglobulins
5 different classes of serum proteins that served as anti globulins and antibodies in the body
- IgG
- IgA
- IgE
- IgD
- IgM
The most common antibody used in immunohistochemistry
IgG
It is the structural part of the antigen that reacts with an antibodie
Epitope
It is a group of amino acids in globular proteins and sugar side chains in polysaccharides
Epitope
