lec 29 eukaryotic transcription cont.

Question 1

what happens to pre-RNA in eukaryotic transcription?

Answer 1

it gains a 5’ cap and a 3’ tail and then is spliced into mRNA

Question 2

beyond what does RNAP II synthesize mRNA?

Answer 2

a cleavage signal sequence

Question 3

what proteins bind to the cleavage signal sequence

Answer 3

endonuclease, polyadenylate polymerase, and more.

Question 4

why is mRNA processed in the nucleus?

Answer 4

to remove intron sequences (splicing) (introns and exons are removed while coding regions stay)

Question 5

why does splicing occur in RNA but not DNA?

Answer 5

because RNA has a OH on the 2’ carbon

Question 6

what do small nuclear ribonucleoproteins (snurps) do?

Answer 6

help in removing introns from mRNA

Question 7

is self splicing possible?

Answer 7

yes because RNA is flexible ans can fold on itself

Question 8

what does self splicing require?

Answer 8

pre-mRNA, Mg2+, and free G (GDP, GTP, GP etc.)

Question 9

what does alternative splicing do?

Answer 9

helps in generating protein diversity

Question 10

what is an example of alternative splicing ?

Answer 10

the tropomyosin gene