lec 26 transcription

Question 1

what is RNA made from?

Answer 1

made from DNA template by RNA polymerase

Question 2

does more RNA abundance = more function?

Answer 2

yes

Question 3

can RNA bind to DNA?

Answer 3

Yes because RNA is single stranded and its base pairs are free

Question 4

how do you regulate RNA synthesis?

Answer 4

By controlling RNA polymerase

Question 5

What factors does mRNA concentration depend on?

Answer 5

rate of RNA synthesis and RNA degredation

Question 6

what does gene control consist of?

Answer 6

initiation, degredation and termination

Question 7

what things are needed for RNA synthesis?

Answer 7

DNA template, RNTP, RNA polymerase, Mg2+ cofactor

Question 8

what is adenovirus?

Answer 8

a virus that makes transcription occur in both directions, rather than just 5’-3’

Question 9

where does RNA polymerase bind too?

Answer 9

the promoter region

Question 10

what are -10 (TATAAT) and -35 (TTGACA) regions?

Answer 10

sigma factors that lead RNA polymerase to +1 region to start transcription

Question 11

what are the two types of transcription?

Answer 11

rho-dependent or rho-independent

Question 12

what is rho-independent termination?

Answer 12

makes hairpin loop rich with G-C to terminate transcription via weak U-A bonds which break off

Question 13

what is rho-dependent termination?

Answer 13

has a termination sequence and upstream from it there is the rut which is C-A rich, the helicase migrates up towards it and it causes it to disrupt the RNA-DNA base pairing