lec 28 eukaryotic transcription

Question 1

why is transcription and translation not coupled in eukaryotes?

Answer 1

because transcription occurs in the nucleus

Question 2

how many RNA polymerases do eukaryotes have?

Answer 2

3

Question 3

how were the 3 RNA pol discovered?

Answer 3

by using alpha-amanitin which they are sensitive too

Question 4

what does alpha-amanitin do?

Answer 4

it stops RNA pol II during elongation

Question 5

why do eukaryotic promoters have different DNA sequences?

Answer 5

to recruit different RNA pol’s

Question 6

what does TF stand for?

Answer 6

transcriptional factors

Question 7

what does RNA pol II make?

Answer 7

mRNA’s

Question 8

what do TF’s do?

Answer 8

they are proteins that help in transcribing DNA

Question 9

what is TBP?

Answer 9

a subunit of TFIID

Question 10

what does TBP contain and where does it bind?

Answer 10

TATA binding protein and it binds to the minor groove of the TATA boxes

Question 11

why is positive regulation of eukaryotic transcription so prominent?

Answer 11

because it allows Tf’s to help in transcription

Question 12

what is required for initiation of transcription to occur

Answer 12

activators

Question 13

what do mediator proteins do?

Answer 13

allow the activators to communicate with RNAP II and TFs