Labs Flashcards
blood collected in tubes with no anticoagulant must be what
left for one hour for the blood to clot
what are the two types of anticoagulant tubes
lavender : EDTA
green : heparin
to obtain serum or plasma, what must happen to blood collected in tubes with no anticoagulant
blood samples must be centrifuged to seperate out the cellular components of the blood
explain the layers from in tube after centrifuging
bottom layer containing red blood cells
middle layer containing mostly white blood cells
top straw coloured layer containing either plasma or serum
if blood was collected with anticoagulant, what will the layers look like
there is a middle layer of white cells and platelets called the “buffy coat”
and the top layer is called plasma
if blood was collected without anticoagulant, what will the layers look like
the straw coloured liquid at the top is called serum
a C reactive protein cut off of what is usually used to indicate inflammation or infection when interpreting plasma ferritin data
> 10mg/L
explain whole blood and how you would collect it
blood that has not been separated from its components, collected with anticoagulant and not separated
explain plasma and how you would collect it
liquid proportion of blood that remains after RBC, WBC and platelets have been removed
collect with anticoagulant and centrifuge blood to separate cellular components
what is serum and how would you collect it
liquid portion of the blood that remains once the clot is removed
collected in tube without anticoagulant, often containing clot activator
blood left to sit for an hour
blood centrifuged to remove clot from serum
what does serum not contain that plasma does
serum does not contain clotting agents as they have been used in the clotting process
does not contain fibrinogen
explain what a blood sample taken from a lavender or green top tube would look like
they contain anticoagulant so the blood wouldn’t clot
RBC at the bottom
WBC and platelets in the middle = called the “buffy coat”
plasma on top
explain what a blood sample taken from a red or gold top tube would look like
contain clotting agent so the blood would clot
blood would be divided into the clot and serum
what does a complete blood count consist of
- haemoglobin
- haematocrit
- red cell count
- platelet count
- the number of white blood cells
- three red cell indices : mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration
vitamin B12 and folate deficiencies are associated with what type of anaemia
macrocytic, normochromic anaemia
