Labs 1-5 Flashcards
appropriate technique for labeling and incubating petri plates
Label the BOTTOM with: Incubation time, temp, date, group #, initials, sample; incubate plates upside down
broth to broth uses
loop
slant to slant uses
loop
plate to slant uses
needle
broth to slant uses
loop
describe aseptic technique for transfers
flame loop, cool; remove lid of tube, flame tube, dip loop, flame tube, replace cap; inoculate sample, flame loop
how to calculate total magnification
ocular (always 10) X objective Ex: 100x lens has total magnification of 100 X 10= 1000
3 basic shapes of bacteria
bacilli (rods); cocci (round); spirals
arrangements (and descriptions) of cocci (6 types)
singular, diplo (2), strepto (chain form), staphylo (grape-like cluster), tetrad (grp of 4), sarcina (packet of 8)
arrangements (and descriptions) of bacilli (3 types)
singular, diplo (2), strepto (chain form)
3 types of spiral bacteria
vibrio (comma shaped curved rod), spirillium (twisted rod), spirochetes ( cork screw)
spirochaetes are motile using _____
axial filaments
what happens to the D value as you lower the NA?
As you lower the NA, the D value gets bigger
What happens to the D value as the wavelength goes up?
as the wavelength goes up, the D value gets bigger
how to calculate D (resolving power)
D= wavelength (0.5)/ NA
what is the limit of resolution for any light microscope
0.2 micrometers
what human disease is often diagnosed using darkfield microscopy
syphilis
what bacterial morphology is best identified using darkfield microscopy
spiral
formula to calculate ocular micrometer issues
(sm/om) X (distance between stage divisions in mm) then convert to microns
sm= stage micrometer om=ocular micrometer
