Laboratory techniques Flashcards
Personal safety
Consistent use of safety goggles, laboratory coat, close toed shoes, gloves, fume hood, facemask or rest or respirator
Accuracy
A measurement giving the actual value for the sample with a limited deviation
Precision
The repeatability of a set of measurements on the same sample
Glassware
Used because of its strength, inertness, transparency and heat resistance
Non-calibrated glassware
Test tubes, speakers, Erlenmeyer flask, round bottom flask, transfer/pasture pipettes, petri dishes, watch glasses, bottles, jars and vials. Volume markings are approximate and they should not be used for actual measurements
Calibrated glassware
Graduated cylinder, buret, graduated pipette, volumetric pipette, volumetric flask. Used for any laboratory measurement that requires accuracy and precision. Manufactured to provide exact volume at a given temperature, 25°C
pH meter
Determines the acidity or basic city of a solution by comparing the voltage (electrical potential) produced by the solution compared to the voltage of a known standard solution and uses the difference in voltage (the potential difference) between them to calculate the pH
Standard laboratory balances/top loading balances
Used when weights are in the milligram range and require accuracy to within plus or minus 0.5 mg
Analytical balances
Used for sensitive weighing applications with accuracy to plus or minus 0.01 mg