Laboratory Techniques

Question 1

Affinity chromatographic separation

Answer 1

The displacement of the protein from the column in this tep involved disrupting the binding of the (His)6 tag to the column

Question 2

Size-exclusion chromatography

Answer 2

Separate based on molecular weight, not selective column binding

Question 3

Cation-exchange chromatography

Answer 3

Separates proteins with different positive charges (or positive vs negative/neutral); its separation method is not based on selective binding to the column, it is based on a functional group-specific chemical rxn or ligand-receptor interaction

Question 4

Anion-exchange chromatography

Answer 4

Separates proteins with different negative charges (or negative vs positive/neutral); its separation method is not based on selective binding to the column, it is based on a functional group-specific chemical rxn or ligand-receptor interaction