Laboratory: Stool Culture Techniques Flashcards
The process of growing microorganisms / parasites in culture by taking them from the infection site by some means of specimen collection and growing them in the artificial environment of the laboratory
Cultivation
Microbes/parasites that grow and multiple in or on a culture medium.
Culture
A medium for growing microorganisms or parasites
Culture medium
Is culture routinely done for parasite identification?
No, only employed for accurate identification
Patients who took anti-parasitic or anti-helminthic drugs can still have their fecal specimen cultured, True or False?
False
An egg slant with an overlay of sterile serum or liver extract in buffered saline
Boeck and Drbohlav medium
Uses a chick embryo extract liquid overlay on a nutrient agar slant
Diamond
Egg yolk-liver extract infusion medium
Balamuth’s monophasic liquid medium
Nutrient Broth
What is the live bacteria used as nutrient for N. fowleri in culture?
E. coli
NNN medium is used for what protozoa?
Leishmania
Trypanosoma
What blood is used in NNN?
Rabbit blood
NNN medium means?
Novy-Macneal-Nicolle
Much more recommended in IV culture for Leishmania
Schneider’s insect tissue culture medium
What specie of fly is found as cell in Schneider’s medium?
Drosophila melanogaster
Culture Methods for Helminths
Harada-Mori
Slant Culture
Agar Plate Cultivation
Baermann Funnel Technique
Charcoal Culture
Useful for the detection of infection with Hookworm, Strongyloides and Trichostrongylus
Harada-Mori
In Harada-Mori, water should be _ the fecal mass?
Below
How many minutes of immersion in boiling water for Harada-Mori?
15 minutes
After the _ day, it will develop into rhabditiform larvae. On the _ day, the hookworm eggs will have developed into filariform larvae
5th
10th
Technique that allows the visualization of motile larvae
Slant Technique
More sensitive method than direct fecal smear of concentration techniques
Agar Plate Cultivation
How do you identify in Agar Plate Cultivation when it is positive?
Visible tracks of crawling larva
Can also be used to examine soil specimens for the presence of larvae with the use of funnel
Baermann Funnel
How many parts of moistened charcoal is used in Charcoal Culture?
5-10 parts
Short buccal cavity; Prominent genital primordium
S. stercoralis L1
Longer esophagus than Hookworm; Notched tail
S. stercoralis L3
Short and stout; open mouth; feeding stage
Hookworm L1
Sheathed, pointed tail, closed mouth; non-feeding stage
Hookworm L3
Semilunar cutting plates are found in what parasite?
N. americanus
2 pairs of ventral teeth is found in what parasite?
A. duodenale
