Laboratory: Stool Culture Techniques

Question 1

The process of growing microorganisms / parasites in culture by taking them from the infection site by some means of specimen collection and growing them in the artificial environment of the laboratory

Answer 1

Cultivation

Question 2

Microbes/parasites that grow and multiple in or on a culture medium.

Answer 2

Culture

Question 3

A medium for growing microorganisms or parasites

Answer 3

Culture medium

Question 4

Is culture routinely done for parasite identification?

Answer 4

No, only employed for accurate identification

Question 5

Patients who took anti-parasitic or anti-helminthic drugs can still have their fecal specimen cultured, True or False?

Answer 5

False

Question 6

An egg slant with an overlay of sterile serum or liver extract in buffered saline

Answer 6

Boeck and Drbohlav medium

Question 7

Uses a chick embryo extract liquid overlay on a nutrient agar slant

Answer 7

Diamond

Question 8

Egg yolk-liver extract infusion medium

Answer 8

Balamuth’s monophasic liquid medium
Nutrient Broth

Question 9

What is the live bacteria used as nutrient for N. fowleri in culture?

Answer 9

E. coli

Question 10

NNN medium is used for what protozoa?

Answer 10

Leishmania
Trypanosoma

Question 11

What blood is used in NNN?

Answer 11

Rabbit blood

Question 12

NNN medium means?

Answer 12

Novy-Macneal-Nicolle

Question 13

Much more recommended in IV culture for Leishmania

Answer 13

Schneider’s insect tissue culture medium

Question 14

What specie of fly is found as cell in Schneider’s medium?

Answer 14

Drosophila melanogaster

Question 15

Culture Methods for Helminths

Answer 15

Harada-Mori
Slant Culture
Agar Plate Cultivation
Baermann Funnel Technique
Charcoal Culture

Question 16

Useful for the detection of infection with Hookworm, Strongyloides and Trichostrongylus

Answer 16

Harada-Mori

Question 17

In Harada-Mori, water should be _ the fecal mass?

Answer 17

Below

Question 18

How many minutes of immersion in boiling water for Harada-Mori?

Answer 18

15 minutes

Question 19

After the _ day, it will develop into rhabditiform larvae. On the _ day, the hookworm eggs will have developed into filariform larvae

Answer 19

5th
10th

Question 20

Technique that allows the visualization of motile larvae

Answer 20

Slant Technique

Question 21

More sensitive method than direct fecal smear of concentration techniques

Answer 21

Agar Plate Cultivation

Question 22

How do you identify in Agar Plate Cultivation when it is positive?

Answer 22

Visible tracks of crawling larva

Question 23

Can also be used to examine soil specimens for the presence of larvae with the use of funnel

Answer 23

Baermann Funnel

Question 24

How many parts of moistened charcoal is used in Charcoal Culture?

Answer 24

5-10 parts

Question 25

Short buccal cavity; Prominent genital primordium

Answer 25

S. stercoralis L1

Question 26

Longer esophagus than Hookworm; Notched tail

Answer 26

S. stercoralis L3

Question 27

Short and stout; open mouth; feeding stage

Answer 27

Hookworm L1

Question 28

Sheathed, pointed tail, closed mouth; non-feeding stage

Answer 28

Hookworm L3

Question 29

Semilunar cutting plates are found in what parasite?

Answer 29

N. americanus

Question 30

2 pairs of ventral teeth is found in what parasite?

Answer 30

A. duodenale