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Clinical Parasitology > Laboratory: Examination of Parasites in Blood Specimen > Flashcards

Laboratory: Examination of Parasites in Blood Specimen Flashcards

1
Q

Capillary blood should be free-flowing and not contaminated with the alcohol used to cleanse the puncture site. True or False?

A

True

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2
Q

Anticoagulants do not cause distortion to the staining process and subsequent. True or False?

A

False, it does cause some distortion.

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3
Q

If malaria is suspected, it is best to prepare smears within _ hour of collection

A

1 hour

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4
Q

Methods of Blood Examination for Venous Blood Sample

A

Knott’s Concentration Method
Membrane Filtration Method

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5
Q

Methods of Blood Examination for Finger-Prick Blood Sample

A

Wet/Fresh Preparation
Capillary Tube Method
Stained Smears

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6
Q

This method is designed to concentrate blood specimens suspected of containing low numbers of microfilariae

A

Knott’s Concentration Method

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7
Q

Supernatant is discarded and sediment is smeared and stained with Wright stain in Knott’s Concentration Method. True or False?

A

False, it is stained with Giemsa

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8
Q

How many mL of blood is needed in Membrane Filtration Method?

A

1 mL

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9
Q

How many mL of distilled water is added in Membrane Filtration Method?

A

10 mL

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10
Q

What is the filter used where the combined blood and distilled water pass through?

A

Swinney filter

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11
Q

What stain is used in Membrane Filtration Method?

A

Dilute Giemsa

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12
Q

Types of membrane filters used for MFM?

A

Millipore
Nucleopore

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13
Q

What is the most sensitive method?

A

Membrane Filtration Method

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14
Q

Species identification is not possible in what method?

A

Wet or Fresh Preparation

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15
Q

In Buffy Coat Film, what is the color of the nuclear material and cytoplasm of L. donovani?

A

Dark red-purple
Light blue

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16
Q

What can be detected in large mononuclear cells found in buffy coat?

A

L. donovani
Trypanosomes
Histoplasma capsulatum

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17
Q

What is the pre-coating of capillary tube in QBC?

A

Acridine orange
Potassium oxalate

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18
Q

What can be found in buffy coat?

A

Lymphocytes
Monocytes
Granulocytes
Platelets

19
Q

This is the stain of choice because it provides optimal detail of intracellular malarial parasites

A

Giemsa stain

20
Q

This stain already contains alcohol so fixation is not required before staining

A

Wright’s stain

21
Q

This stain is used to improve visibility of the microfilarial sheath

A

Delafield’s Hematoxylin stain

22
Q

Percentage of formalin and acetic acid that dehemoglobinize thick films?

A

2% formalin
1% acetic acid

23
Q

If whole blood is used: examiner should continue stirring about _ seconds to prevent the formation of fibrin strands

A

30 seconds

24
Q

Are thick films used for screening purposes? True or False?

A

True

25
Q

This smear is used routinely used for parasite identification to the species level

A

Thin Films/Smear

26
Q

Name of method done using two clean glass slides to produce thick on one end and thin and feathery at the other end

A

Wedge method
Push slide technique

27
Q

Condition of blood where the morphology of both parasites and infected RBCs may not be typical

A

Anticoagulated blood from an hour ago

28
Q

Which is examined first, thin smear or thick smear?

A

Thin smear

29
Q

pH level of buffer used in Giemsa staining

A

pH 7.0-7.2

30
Q

Ratio of Giemsa to buffer

A

1 part Giemsa stock : 10-50 parts buffer

31
Q

Lack of _ fixation allows for lysis of red cells in the Giemsa staining solution.

A

Methanol

32
Q

How many fields before a film is reported as negative?

A

100-300 fields
Preferred is 100, only 300 for non-immune patients

33
Q

If 200 leukocytes were counted; no. of parasites counted is multiplied by?

A

40

34
Q

if 500 leukocytes were counted; no. of parasites counted is multiplied by?

A

16

35
Q

Color of Leishmania, trypanosome, malaria and Babesia nuclear structures when stained

A

Red

36
Q

Color of cytoplasm when stained

A

Blue

37
Q

Color of Schiffer’s dot when stained

A

Red

38
Q

Color of nuclei when stained

A

Blue to purple

39
Q

Color of sheath when stained

A

Clear, may not stain

40
Q

Color of RBC when stained

A

Pale red

41
Q

Color of WBC when stained

A

Purple

42
Q

Color of neutrophilic granules when stained

A

Pink-purple

43
Q

Color of eosinophilic granules when stained

A

Purple-red

44
Q

Smear used for screening parasites, detecting mixed infections

A

Thick Film/Smears

Clinical Parasitology (13 decks)

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