Laboratory Safety and Sample Collection Flashcards

1
Q

“Treat all sample infectious”

A

Standard Precaution

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2
Q

“All sample can induce bloodborne pathogens”

A

Universal Precaution

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3
Q

Potential risks in parasitology laboratory

A
  • Ingestion of eggs/ova
  • Skin penetration of infective larva
  • Infection of non-parasitic agents
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4
Q

True or false. Fixed stool using specimens are already free from pathogens.

A

False. They are still infectious.

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5
Q

When should blood specimens be collected?

A

Before treatment is initiated

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6
Q

Capacity of fecal sample container

A

0.5 pint or approx. 240 ml

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7
Q

Labile stage and feeding form of protozoans

A

Trophozoites

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8
Q

How many stools samples should be collected and how frequent?

A

3 specimens collected within 6-7 days (no more than 7 days)

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9
Q

How many stools samples should be collected for intestinal amebiasis and how frequent?

A

No more than 6 specimens collected within 10 days

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10
Q

How many weeks of deferment if the patient is taking antiprotozoan?

A

3-4 weeks

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11
Q

How many weeks of deferment if the patient is taking helminthic drugs?

A

5-6 weeks

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12
Q

Drugs that require 1 week deferment

A

o Antacids
o Anti-diarrheal
o Barium
o Bismuth
o Laxatives

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13
Q

Why does the intake of antibiotic affect the result of stool analysis?

A

They decrease the number of protozoan for several weeks

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14
Q

Amount of stool to be collected

A

Thumb-sized (solid)
5-6 tablespoons (watery)

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15
Q

Trophozoites are likely to die within _____ after passage

A

30 minutes to 1 hour

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16
Q

Acceptable temperature of refrigerator for stool samples

A

3-5C (most optimal = 4C)

17
Q

An all-purpose fixative that can be an eye irritant

18
Q

Concentration of formalin recommended for protozoan cysts

19
Q

Concentration of formalin recommended for helminth eggs and larva

20
Q

When using formalin as a fixative, the stool must be fixed within how many minutes?

A

30 minutes

21
Q

This fixative has Mercuric chloride in its stock solution, which is why it is often regarded as a dangerous fixative

A

Schaudinn’s solution

22
Q

Best option for permanent staining

A

Mercuric chloride

23
Q

A plastic resin which serves to adhere a stool sample onto a slide

A

Polyvinyl alcohol (PVA)

24
Q

Main advantage: related to the preservation of protozoan cysts and trophozoites for permanent staining

A

Polyvinyl alcohol (PVA)

25
True or false. For modified PVA, copper is preffered over zinc
False
26
Function of iodine in Merthiolate-Iodine-Formalin (IMF)
Stain
27
Useful for the fixation of intestinal protozoans, helminth eggs, and larvae
Merthiolate-Iodine-Formalin (IMF)
28
Not suitable for permanent smears stained with trichrome
Merthiolate-Iodine-Formalin (IMF)
29
Interferes with other stains and fluorescence; may cause distortion of protozoa
Iodine in IMF
30
Has glacial acid that helps lyse the cells and albumin-glycerol which sticks the sample to the slide
Sodium acetate-acetic acid formalin (SAF)
31
What fixatives can be used for direct wet mount preparation?
Formalin, MIF, SAF
32
What fixatives can be used for concentration procedures?
Formalin, MIF, SAF
33
What fixatives can be used for permanent staining?
Schaudinn's solution, PVA, Modified PVA, SAF
34
Predominant stage in watery sample
Trophozoites (processed within 30-60 mins)
35
Predominant stage in formed sample
Cyst (may be processed for up to 24 hours)