Laboratory Safety and Basic Infection Control

Question 1

Personal Safety Guidelines

Answer 1

No Food, Drink, Gum Chewing
No loose clothing, dangling jewelry, long hair must be tied back
Wear appropriate lab attire (closed-toe shoes, safety glasses, etc.)

Question 2

Fire Classification

Answer 2

Class A:	Ordinary combustibles
     (Ash)
Class B:	Flammable liquids & gases
     (Boil)
Class C:	Electrical Equipment
    (Cord)

Question 3

RACE

Answer 3

Rescue
Alarm
Contain
Extinguish

Question 4

PASS

Answer 4

Pull Pin
Aim Nozzle
Squeeze handle
Sweep side to side

Question 5

Avoid Electrical Shock

Answer 5

Equipment inspections
Avoid extension cords
Don’t handle electrical equipment if floor or feet are wet
Avoid contact with electrical equipment while drawing blood
Always unplug equipment BEFORE performing any maintenance

Question 6

3 Principles to Radiation Exposure

Answer 6

Time
Shielding
Distance

Question 7

Safety Data Sheets

Answer 7

Required by OSHA
Associated with each chemical
Provides:
Product or chemical identity
Contact for hazard information
Exposure limits
Physical and chemical characteristics
Physical hazards
Cleanup procedures
Emergency and first aid procedures

Question 8

Safety Measures

Answer 8

Flush area for 15 MINUTES
Wear goggles and work in a hood or near a sink
Always add acid to base
Always hold a bottle by the body, never the neck.

Question 9

Department of Transportation Label

Answer 9

Hazard class symbol
UN specific four digit ID number
UN hazard class number

Question 10

Biological Hazards

Answer 10

Any material of substance harmful to health
Common routes of entry
Ingestion
Skin contact
Airborne

Question 11

Types of biohazards material

Answer 11

Viral
Fungal
Bacterial

Question 12

Protection and prevention

Answer 12

Gloves
Lab coat
Mask
Face shield
Disposal
Sharps containers
Biohazard waste container
Disinfectant
10% bleach: bactericidal, viricidal
5% amphyl: bactericidal

Question 13

Waste

Answer 13

Three types of waste
Uncontaminated paper waste
Contaminated paper waste
Sharps

Question 14

Regular waste basket

Answer 14

Any paper product not visibly contaminated with blood or body fluids
Examples:
Magazines
Paper towels
Requisitions

Question 15

Biohazard Waste

Answer 15

Waste visibly contaminated with blood 
    or body fluids.
Examples
Blood soaked gauze or cotton balls
Paper towels soiled with blood

DO NOT throw ordinary waste into these containers. Contents are decontaminated first

Question 16

Sharps

Answer 16

Any item that can puncture of scrap the skin
Needles
Lancets
Glass
Sharps containers are rigid puncture proof containers
Used needles are designed to be disposed of as a unit

Question 17

Isolation

Answer 17

Trash from an isolation room must be disposed of using a double-bagging procedure
The contaminated materials are sealed in the first bag inside of the room.
A second person outside the room seals the bag in another bag and seals it.

Question 18

Centrifuge

Answer 18

Separates solutions by centrifugal force

Safety
Close lid
Use aerosol cover
Balance items according to weight
DO NOT STOP by hand, allow it to stop on its own
Do not use cracked tubes
Clean centrifuge if breakage has occurred

Question 19

Patient Safety

Answer 19

Safety rules
No Running in the hallways
Be aware of your surroundings
Check for spilled liquids
Keep venipuncture equipment away from patient
Clean up after yourself
Avoid touching electrical equipment during a draw
Report any patient problems to the nurse
Replace bedrails in the upright position when you are finished

Question 20

Latex allergies

Answer 20

Always check for a posted sign indicating a patient has a latex allergy. 
Do not use latex gloves or tourniquets
LLUMC is a latex free facility
Signs and symptoms of an allergic reaction
Rash/itching
Hives
Eye and sinus irritation
Shock