Laboratory - Lipids Flashcards
What is the positive color of ninhydrin test?
Blue-violet solution
Mainly used to detect the presence
of cephalins, lecithins, and sphingomyelins
Ninhydrin Test
Mainly used in detecting the presence of
choline-containing substances (glycerophosphatides, sphingolipids, and phosphorylated lipids)
Kraut’s Test
What is the positive color of Kraut’s Test?
Formation of red-orange solution indicates the presence of choline
What is the positive color of Molish Test?
Purple-colored ring
Based on the dehydration of pentoses to yield furfural or hexoses to 5-hydroxymethyl furfural
Molisch Test
Based on the reaction of hydroxyl group (-OH) of
cholesterol reacting with the reagent (acetic anhydride + conc. sulfuric acid
Liebermann-Burchard Test
Also used to detect the presence of steroids
Liebernmann-Burchard Test
What is the positive color of Liebernmann-Burchard Test?
Bluish-green color
Formation of yellow to brick red colour indicates the presence of cholesterol
Salkowski’s Test
What is the positive color of Salkowski’s Test?
Two colors observed:
Red color in the upper layer (chloroform) and;
green fluorescence in the bottom layer due to the
heavier sulfuric acid settling
A test specific in detecting the presence of
cholesterol in a sample
Salkowski’s Test
What are the general tests for lipids?
- LIEBERMANN-BURCHARD TEST
- KRAUTS TEST
- SALKOWSKI TEST
- NINHYDRIN TEST
- MOLISCH TeST
- ACROLEIN TEST
There are diffrent complex lipids that can be found in the pig’s brain such as?
- Cholesterol
- Phosphatides
- Cerebrosides
- Sphingosines
A pig’s brain contains a high amount of lipid content, the DHA or popularly known as
Omega 3-fatty acid
- Used to determine the iodine value of fats
and oils and thus estimate the unsaturation - The higher the iodine value, the more
unsaturated fatty acid bonds are present in a fat. - Iodine value or number refers to the number of grams of iodine consumer 100g of fat.
- the reaction is based on the ability of unsaturated fatty acids to undergo halogenation
Test for unsaturation
One of the most abundant biomolecule found in living organism
Lipids
A test used to detect the presence of glycerol or fats
Acrolein Test
Detection of a punget, irritating other confirms the presence of glycerol/fats in the sample
Acrolein Test
