Laboratory: Lab Safety Flashcards
Two Primary Causes of Accidents
Unsafe activities
Unsafe environmental condition
Safety Equipment
Safety showers & eye wash station
Fire extinguisher
Fume hood
Biosafety cabinets
Personal Protective Equipment
Refers to biological substances that pose a threat to the health of living organisms, primarily that of humans.
Biological Hazard
Healthcare facilities developed procedures
to control and monitor infections occurring within the facilities
Infection Control
Blood and body fluid precautions should be consistently used for all patients
CDC → Universal Precautions (1985)
Consist of bacteria, fungi, parasites, and viruses
Infectious Agents
The location of potentially harmful microorganisms, such as a contaminated clinical specimen or an infected patient.
Reservoir
Equipment and other soiled inanimate objects will serve as reservoirs, particularly if they contain blood, urine, or other body fluids
Fomites
This can be through the mucous membranes of the nose, mouth, and eyes, and in blood or other body fluids
Portal of Exit
Unprotected host touches the patient, specimen, or a contaminated object
Direct Contact
Inhalation of dried aerosol particles circulating on air currents or attached to dust particles
Airborne
The host inhales material from the reservoir
Droplets
Ingestion of a contaminated
substance
Vehicle
From an animal or insect bite
Vector
Can be the same as the portal of exit, which includes the mucous membranes of the nose, mouth, and eyes, breaks in the skin, and open
wounds
Portal of Entry
Can be another patient during invasive procedures, visitors, and healthcare personnel when exposed to infectious specimens or needlestick injuries
Susceptible Host
Proper hand hygiene, correct disposal of contaminated materials, and wearing personal protective equipment (PPE) are of major importance in the laboratory
Chain of Infection
Daily exposure to blood and body fluids
Category I
Regular exposure to blood and body fluids
Category II
No exposure to blood and body fluids
Category III
Employers must offer HBV vaccine to all personnel (Category I and II)
True
Specimens should be “capped” during centrifugation
True
Any blood, body fluid, or other potentially infectious material spill must be cleaned up using:
Spill cleanup kit
Common aqueous detergent
10% bleach using appropriate contact time
When skin contact occurs, the best first aid is to flush the area with large amounts of water for at least 15 minutes and then seek medical attention
Chemical Spills and Exposure
Chemicals should never be mixed together unless specific instructions are followed, and they must be added in the order specified
Chemical Handling
OSHA also requires all facilities that use hazardous chemicals to have a written
Chemical Hygiene Plan
Classified according to flash point → the temperature at which sufficient vapor is given off to form an ignitable mixture with air
Flammable/Combustible Chemicals
Injurious to the skin or eyes by direct contact or to the tissue of the respiratory and
gastrointestinal tract if inhaled or ingested
Corrosive Chemicals
Spontaneously explode or ignite or that evolve heat or flammable or explosive gases
Reactive Chemicals
Hazardous chemicals should be labeled with a description of their particular hazard, such as poisonous, corrosive, flammable, explosive, teratogenic, or carcinogenic
Chemical Labelling
Properties of MSDS
Physical and chemical characteristics
Fire and explosion potential
Reactivity potential
Health hazards and emergency first aid procedures
Methods for safe handling and disposal
Primary routes of entry
Exposure limits and carcinogenic potential
R.A.C.E.
Rescue
Alarm
Contain
Extinguish/Evacuate
Hazardous material symbol
NFPA 704
Blue in Hazard is
Health Hazard
Red in Hazard is
Fire Hazard
White in Hazard is
Specific Hazard
Yellow in Hazard is
Reactivity
Fire Type composed of wood, paper and clothing
Class A
Fire Type composed of flammable organic materials
Class B
Fire Type composed of electricals
Class C
Fire Type composed of combustible materials
Class D
Fire Type composed of grease, fat and oils
Class K
Extinguisher with material of water
Class A
Extinguisher with material of dry chemicals, carbon dioxide, halon and foam
Class B and C
Extinguisher with material of liquid designed to prevent splashing and cool the fire
Class K
P.A.S.S.
Pull Pin
Aim Nozzle
Squeeze Trigger
Sweep Nozzle
All sharp objects must be disposed in
Puncture-resistant, leak-proof container with the biohazard symbol
All biological waste (EXCEPT URINE) should be placed in appropriate containers labeled with biohazard symbol
True
(Urine) Decontaminate the sink by
1:5 or 1:10 dilution of
sodium hypochlorite (bleach solution)
Waste Disposal Technique
Incineration
Recycling
Landfill burial
Flushing down the drain
Collected in approved
containers
Flammable solvents
Specially-designed
incinerators
Flammable Material
Landfill
Solid Chemicals
Green Disposal Bin
General Waste
Red Disposal Bin
Infected Plastics
Yellow Disposal Bin
Infected Waste
Blue Disposal Bin
Glassware