Laboratory Evaluation of the Cellular Immune System (RVSP)

Question 0

Solution used to obtain lymphocyte pellets

Answer 0

Ficoll hypaque density gradient soln

Question 1

Identification of lymphocytes is useful in

Answer 1

Immunodeficiency diseases
Lymphoproliferative malignancies
Unexplained infectious diseases
Monitoring of transplants
Acquired immunologic abnormalities (AIDS)

ILUMA

Question 2

3 ways to separate lymphocyte pellets

Answer 2

Nylon wool technique
Using monoclonal Abs (flow cytometry, lymphokwik)
Magnetic beads

Question 3

T lymphocyte enumeration mtd that is manual

Answer 3

Rosette assay

Question 4

Rgnt in rosette assay

Answer 4

Sheep’s RBCs

Question 5

In rosette assay, sheep’s RBC’s attach to

Answer 5

CD2 of T cells

Question 6

How many cells are ctd in Rosette assay?

Answer 6

200 cells

Question 7

Formula for B cells

Answer 7

100 - rosettes

Question 8

Tests for lab eval of Lymphocyte function

Answer 8

Transformation assays

• mitogen-induced blastogenesis
• mixed lymphocyte culture

Cytotoxicity assay

Question 9

Mitogen-induced blastogenesis (MIB) is an assessment of

Answer 9

Proliferative capacity of lymphocytes

Question 10

Incubation period of mitogens in mitogen-induced blastogenesis (MIB)

Answer 10

3-4 days

Question 11

Incubation period of antigens in mitogen-induced blastogenesis

Answer 11

5-10 days

Question 12

Radiolabeled DNA nucleoside used in mitogen-induced blastogenesis

Answer 12

Tritiated thymidine

Question 13

Methods for manual lymphocyte enumeration

Answer 13

Hemocytometry

Rosette assay

Question 14

In MIB, after incubation, the harvest cells are transferred to

Answer 14

Disks of filter paper

Question 15

In MIB, the disks of filter paper are placed in

Answer 15

Scintillation counter

Question 16

MIB method where the response of the entire cell population is measured

Answer 16

Bulk Assay Mtd

Question 17

Mitogens for T cells

Answer 17

Phytohemagglutinin
Concanavalin A (Con A)

Question 18

Mitogens for B cells

Answer 18

Staphylococcal protein A

Pokeweed mitogen

Question 19

Mixed lymphocyte assay (MLC) is a special type of lymphocyte __________ assay

Answer 19

Stimulation

Question 20

Major determinant in MLC

Answer 20

D locus of HLA system

Question 21

Test used to assess the ability of histoincompatible lymphocytes from one indiv to stimulate lymphocytes of another indiv

Answer 21

Mixed lymphocyte culture (MLC)

Question 22

In MLC, ___________ cells are mixed with _____________

Answer 22

Irradiated stimulator cells

Irradiated responder cells

Question 23

Used in MLC for incubation

Answer 23

Cell culture

Question 24

Used in MLC to determine degree of stimulation

Answer 24

Scintillation counter

Question 25

Test used in the selection of compatible donor for a BM transplant

Answer 25

MLC

Question 26

Endpoint of cytotoxicity assay

Answer 26

Cell death

Question 27

Cell death may be due to

Answer 27

- Complement activity (complement-mediated cytotoxicity) | - Direct effect of one cell to another cell (cell-mediated cytotoxicity)

Question 28

Target cell lysis is determined by the release of ________ from the target cell or incorporation of a _________

Answer 28

``` Chromium Vital dye (eosin, trypan blue) ```

Question 29

Substances used in Chromium-release assay for stimulation

Answer 29

Phytogemagglutinin | Labeled 51Cr

Question 30

True/False. Chromium-release assay uses PBMC

Answer 30

True

Question 31

Represents unlysed cells in chromium-release assay

Answer 31

Radioactivity in the cells at the end of the experiment

Question 32

Std technique for detection of Ag-Ab interaction on a cell surface

Answer 32

Dye Viability Exclusion Assay

Question 33

Explain the assay for neutrophilic activation

Answer 33

Stimulatory substances bind to surface receptors to illicit metabolic and structural changes that result to phagocytosis, oxidative burst, locomotion, and degranulation

Question 34

Assay that uses particles that are visible within the cell and can be counted after ingestion

Answer 34

Assay of phagocytosis and endocytosis

Question 35

Target cells for the assay of phagocytosis and endocytosis

Answer 35

Neutrophil | Monocyte

Question 36

In the assay of phagocytosis and endocytosis, target cells are incubated with

Answer 36

Yeast

Question 37

Stain used in the assay of phagocytosis and endocytosis

Answer 37

Giemsa

Question 38

Number of cells counted in the assay of phagocytosis and endocytosis

Answer 38

200 cells

Question 39

Increase in oxidative metab during phagocytosis

Answer 39

Oxidative burst

Question 40

Test used in the assay of enzymatic and intracellular killing

Answer 40

Nitro Blue-Tetrazolium test (NBT)

Question 41

Formed when there is a nmal oxidizing environment

Answer 41

Formazan

Question 42

Color of NBT in oxidized form

Answer 42

Yellow

Question 43

Color of formazan

Answer 43

Deep blue

Question 44

True/False. NBT Test is a quantitative assay

Answer 44

False. It is a SEMIQUANTITATIVE assay

Question 45

Disease where the PMNLs will not turn blue in the NBT test

Answer 45

Chronic Granulomatous Disease

Question 46

Tests the phagocytic function of polymorphonuclear leukocytes (PMNLs)

Answer 46

Nitro Blue-tetrazolium test

Question 47

Opsonin in the serum can be detected by incubating serum with __________ and ____________

Answer 47

Normal neutrophils | Yeast particles

Question 48

Assay of membrane permeability uses _____________

Answer 48

Fluorescein diacetate