Laboratory diagnostic approaches to cardiorespiratory parasites Flashcards
1
Q
What clinical signs can be seen by a pig heavily infested with Ascaris Summ
A
- Failure to thrive
- Coughing
- Abdominal expiratory dyspnea (thumping)
- Pendulous abdomen
2
Q
What are some morphological characteristics of Asceris Summ eggs
A
- Lumpy and thick outer layer
- Brownish to greyish
- Thick pitted shell
- Proteinaceous outer coating
- Ovoid
3
Q
Which stage of development in the life cycle of Ascaris Summ occurs in the intestine
A
L4 to adult
4
Q
What is the principle behind McMaster egg counting
A
It is a rapid/ simple quantitative method for counting parasite eggs in faeces, based on flotation on concentrated salt solution in a counting chamber
5
Q
How do you perform McMaster egg counting
A
- Weigh 1g of faecal sample and add a known amount of flotation fluid, then mix by shaking
- Pour the faecal slurry through a sieve and flush a known amount of flotation fluid through the sieve
- Transfer this sample using a pipette to the 2 McMaster slide chambers taking care to avoid bubbles
- Leave the counting chambers to stand for 5 minutes before examination
Examine the filtrate in the filled chambers under the microscope at 4x followed by 10x magnification - Identify and count all eggs within the engraved area of both chambers
6
Q
What is the calculation used in McMaster egg counting
A
Number of eggs/g faeces= (Number of eggs counted x (60) total volume))/ ((0.3) volume of counted chamber x (1) weight of faeces)
7
Q
What are the level of infection parameters in McMaster egg counting for :
- Light
- Moderate
- Heavy infection
A
Light= 100-2000 Moderate= 2000-7000 Heavy= 7000+
