LABORATORY DIAGNOSIS OF FUNGI Flashcards
True/false
All specimens should be transported in leakproof sterile containers and processed later.
FALSE
later –> ASAP
true/false
Anaerobic transport media or anaerobic containers should always be used for fungi
FALSE
should NEVER be used
true/false
dermatophytes are insensitive to cold temperatures.
false
sensitive to cold temperatures
true/false
If delay is expected within 2h, specimens should be stored at 4 C.
true
A requisition form must include which demographics:
Patient name
Age and sex
Location or address
Physician name
Specific culture site
Date and time of specimen collection
Clinical diagnosis
Any special culture request
true/false
Specimen must have a firmly attached label indicating the patient name, specimen type, date and time of collection
true
optimum temperature of blood and CSF samples
30-37 C
optimum temperature of dermatological specimens
15-30 C
optimum temperature for specimens other than blood, CSF samples, and dermatological samples
4 C
true/false
All work in mycology should be carried out in a certified type 1 laminar airflow BSC especially when dealing with filamentous fungi.
false
type 2
true/false
Yeasts cultures should not be handled on the bench in the same manner that bacterial cultures are routinely handled.
false
it can be handled on the bench
true/false
Screw-cap tubes of slanted media are safer to handle than petri plates.
true
true/false
Petri plates should NEVER be used if Coccidioides immitis is suspected
true
Do not make slide cultures of isolates that may be:
Histoplasma capsulatum
Blastomyces dermatitidis
Coccidioides immitis
Coccidioides posadasii
Paracoccidioides brasiliensis
Talaromyces marneffei
Cladophialophora bantiana
review table on specimen collection!
AYAW MO?
EDI WAG
it is necessary to maximize recovery of fungi in order to
Release fungi enclosed within cells.
Concentrate fungal material in the specimen.
Help to reduce or eliminate bacteria present in contaminated specimens because of the action of mucolytic agents
Give three mucolytic agents
N-acetyl-L-cysteine
5% oxalic acid
Dithiothreitol (Sputolysin2)
What specimen should be pretreated through: Granules should be washed & crushed; other materials
should be centrifuge at 2,000g for 10min?
Abscess, drainage, pus,
granules
what specimen should be pretreated through: Lysis in Isolator tubes and then centrifugation for 30min.
at 3,000g, in a 35o fixed-angle rotor or swing bucket.?
blood, bone marrow
what specimen should be pretreated through: Centrifugation at 2,000g for 10min. or membrane
filtration?
body fluids
what specimen should be pretreated through: Mince; gently pushed pieces down into agar?
nails
what specimen should be pretreated through: Lysis with mucolytic agents, followed by centrifugation
at 2,000g for 10min?
respiratory secretions (BAL, sputum)
what specimen should be pretreated through: Mince or grind in a mortar; push pieces down into agar?
tissue
what specimen should be pretreated through: Centrifugation at 2,000g for 10min?
urine
