Basic Concepts in Mycology 2 Flashcards
basic structural unit consists of a chain of multinucleate, tubular, filament-like cells.
Hypha
Most multicellular fungi in their vegetative state, consists of a mass of branching hyphae called mycelium.
What is the other term for mycelium?
Thallus
Yes or No
does hypha have rigid cell wall?
Yes
the hyphae are divided into compartments or cells by the development of more or less frequent cross-walls, termed as
septa
What do you call a hyphae that has cross walls?
Septate hyphae
Fungi that exists in the form of microscopic multicellular mycelium are commonly referred to as
Mold
This is a type of fungi that exists in the form of independent single cells propagate by budding out similar cells from their surface.
unicellular fungi
A fungi that came from the production of a chain of cells or described as loosely arrangement of budding cells
yeast
continued elongation of the parent cell before it buds out results in a chain of elongated cells, termed as
Pseudohypha
Medically important fungi that change their growth form as a part of the process of tissue invasion.
dimorphic fungi
This is a concept in which multicellular mold form in the natural environment transforms into a single-celled yeast form when invading tissues under the influence of temperature.
Thermal dimorphism.
Temperature of multicellular mold form in the natural environment.
25 - 30 C
Temperature of single-celled yeast form in the tissues.
35 - 37 C
3 examples of dimorphic fungi:
Histoplasma capsulatum
Blastomyces dermatitidis
Coccidioides immitis
Paracoccidioides brasiliensis
Sporothrix schenckii
Part of the fungi that is rigid, mostly composed of polysaccharides, proteins and glycoproteins.
Cell wall
Percentage of polysaccharides, proteins and glycoproteins in the cell wall?
polysaccharides: 90%
proteins and glycoproteins: 10%
What are the polysaccharide present in the fungal cell wall?
chitin, glucan, chitosan, galactosan, and mannan
Chitin is the glycoproteins in various combinations.
Chitin: the major carbohydrate consist of repeating monomers of ___ which provides shape and protection from osmotic lysis (unaffected by some antibiotics)
N-acetyl-glucosamine (NAG)
What are the functions of the fungal cell wall?
Barrier between fungal cell and its external environment.
Binding site for some enzymes
Possesses antigenic properties which allow interaction with other organisms
Part of the fungal cell that is composed of structurally arranged phospholipids in two-layered configuration scattered randomly.
cell membrane
major component of the cell membrane that regulates solute intake and secretion (transport system) through selective permeability and serves as the target of antifungal drugs like Nystatin.
Ergosterol
Part of the fungal cell that is an external coating located outside or covering the cell wall and found only in certain fungi.
Capsule
The capsule is composed of __
amorphous polysaccharide
Part of the fungal cell that contributes to its virulence factor
Capsule
nutritional type of fungi
chemoheterotrophic
fungi is similar to plants but differ in:
they lack chlorophyll
true/false
Fungi are capable of growing on living organic matter but not on dead organic matter.
false
they are also able to grow in dead organic matter (saprophytic)
Fungi are usually nonmotile except for:
Phylum Chytridiomycota and species of Rhizopidium
true/false
fungi are mostly aerobic and some are facultative anaerobic
true
What pathway is used in lysine syntehsis?
alpha-aminodiphate pathway
It describes the propagules that result form an asexual process (mitosis only) and generally short-lived propagules.
Conidium
This is produced from the fusion of two compatible nuclei followed by meiosis.
Spores
In some cases, spores are produced in millions in macroscopic “fruiting bodies” such as
Mushrooms
Fungi vs Bacteria
In terms of cell wall:
Fungi: polysaccharides (i.e. chitin, glucan, mannan, galactosan, and chitosan)
Bacteria: polysaccharide and peptidoglycan
Fungi vs Bacteria
in terms of cell membrane:
Fungi: ergosterol
Bacteria: no sterols except ureaplasma and mycoplasma
Fungi vs Bacteria
in terms of nucleus:
fungi: small, bound by nuclear membrane
bacteria: nucleoid, no nuclear membrane
Fungi vs Bacteria
in terms of chromosome:
fungi: linear
bacteria: circular
Fungi vs Bacteria
in terms of ribosomes:
fungi: 80s
bacteria: 70s
Fungi vs Bacteria
in terms of lysine synthesis:
fungi: alpha-aminodiphate pathway
bacteria: DAP (diaminopimelate pathway)
true or false
yeasts are able to assimilate sugars and nitrate, and ferment sugars
true
identification of yeasts relies on the combination of:
morphological, physiological, and biochemical characteristics
what does (MALDI-TOF)2 mean?
matrix-assisted laser desorption ionization-time of flight mass spectrometry
Macroscopic characteristics of fungi observed:
colonial form, surface color (obverse) pigmentation, reverse pigmentation, and growth rate.