Laboratory Chemicals, Supplies and Equipment Flashcards
Purpose of Clinical Laboratory
To facilitate the correct performance of procedures that yield accurate and precise information, aiding in the patient’s diagnosis and treatment.
Role of Clinical Laboratory Scientist
To correctly use supplies and equipments and have an understanding of the fundamental concepts critical to any procedures.
2 Components of Quantitative Laboratory Result
- Actual value
- Unit
The ____ defines the physical quantity or dimension (ex. mass, length, time, or volume).
unit
The ____ was adopted internationally in 1960, and is preferred in scientific literature and clinical laboratories.
Systeme International d’Unites (SI)
Systeme International d’Unites (SI) was adopted internationally in ____.
1960
Systeme International d’Unites (SI) is based on the ____.
metric system
How many base quantities are there in SI units?
7
Base quantities
- Length (m)
- Mass (kg)
- Time (s)
- Electric current (A)
- Thermodynamic temperature (K)
- Amount of substance (mol)
- Luminous intensity (cd)
Give the name and symbol of the base quantity
Length
Meter (m)
Give the name and symbol of the base quantity
Mass
Kilogram (kg)
Give the name and symbol of the base quantity
Time
Seconds (s)
Give the name and symbol of the base quantity
Electric current
Ampere (A)
Give the name and symbol of the base quantity
Thermodynamic temperature
Kelvin (K)
Give the name and symbol of the base quantity
Amount of substance
Moles (mol)
Give the name and symbol of the base quantity
Luminous intensity
Candela (cd)
____ exist in varying grades of purity.
Analytical Chemicals
Analytical Chemicals exist in varying ____.
grades of purity
Analytical Chemicals
- Analytical reagent (AR)
- Ultrapure (UP)
- Chemically pure (CP)
- United States of Pharmacopeia (USP)
- National Formulary (NF)
- Technical or Commercial grade
Organic Reagents
- Practical grade
- Chemically pure
- Spectroscopic
- Chromatographic
- Reagent grade
Identify which Analytical Chemical is being described
Established by the American Chemical Society (ACS)
Analytical Reagent (AR)
Analytical Reagent (AR) grade is established by ____.
American Chemical Society (ACS)
Identify which Analytical Chemical is being described
Labels on reagents state either the:
* actual impurities
* maximum allowable impurities
* % of impurities
* ACS inital
* For laboratory use
* ACS standard grade reference materials
Analytical Reagent (AR)
Identify which Analytical Chemical is being described
Suitable for use in most analytical laboratory procedures
Analytical Reagent (AR)
Identify which Analytical Chemical is being described
Used for quantitative and qualitative analysis, and for accuracy.
Analytical Reagent (AR)
Identify which Analytical Chemical is being described
Used for trace metal analysis
Analytical Reagent (AR)
Identify which Analytical Chemical is being described
Used in the preparation of standard solutions
Analytical Reagent (AR)
Identify which Analytical Chemical is being described
These are reagents that have been put through an extra step or additional purification step
Ultrapure (UP)
Identify which Analytical Chemical is being described
Used in specific procedures such as:
* Chromatography
* Atomic Absorption
* Immunoassays
* Molecular diagnostics
* Standardization
* Other techniques that require extremely pure chemicals
Ultrapure (UP)
Ultrapure (UP) reagents are used in the following procedures such as ____.
- Chromatography
- Atomic Absorption
- Immunoassays
- Molecular diagnostics
- Standardization
- Other techniques that require extremely pure chemicals
Identify which Analytical Chemical is being described
Used to manufacture drugs
- United States of Pharmacopeia (USP)
- National Formulary (NF)
Identify which Analytical Chemical is being described
Limitations established for this group of chemicals are based only on the criterion of not being injurious to individuals.
- United States of Pharmacopeia (USP)
- National Formulary (NF)
Identify which Analytical Chemical is being described
Approved for human consumption
- United States of Pharmacopeia (USP)
- National Formulary (NF)
Identify which Analytical Chemical is being described
Pure enough for use in most chemical laboratories, however, purity standards are not based on the needs of the laboratory.
- United States of Pharmacopeia (USP)
- National Formulary (NF)
Identify which Analytical Chemical is being described
The impurity limitations are not stated
Chemically Pure (CP)
Identify which Analytical Chemical is being described
Preparation of these chemicals are not uniform
Chemically Pure (CP)
Identify which Analytical Chemical is being described
Purity is delivered by measurement of the melting point or boiling point
Chemically Pure (CP)
Identify which Analytical Chemical is being described
Not recommended for reagent preparation unless further purification step or a reagent blank is included.
Chemically Pure (CP)
Identify which Analytical Chemical is being described
Used in manufacturing
Technical or Commercial Grade
Identify which Analytical Chemical is being described
Should never be used in the clinical laboratory
Technical or Commercial Grade
Reference Materials
- Primary Standard
- Secondary Stadard
These are highly purified chemicals measured directly to give an exact concentration of purity which can be use for comparison.
Primary Standard
The ____ are used instead of ACS primary standard materials.
National Institute of Standards and Technology-certified Standard Reference Materials/Certified Reference Materials (NIST SRMs)
A substance of lower purity with concentration that is determined by comparison with the primary standard.
Secondary Standard
Categories of Water
- Clinical Laboratory Reagent Water (CLRW)
- Special Reagent Water (SRW)
- Instrument Feed Water
- Water supplied by method manufacturer
- Autoclave and wash water
- Commercially bottled purified water
Determine which type of water purity
Used for test method requiring minimum interference such as trace metal, iron, and enzyme analysis.
Type I
Determine which type of water purity
Used for procedures needing maximum water purity
Type I
Determine which type of water purity
Consumed immediately after it is produced
Type I
Determine which type of water purity
Acceptable for most analytic requirements, including reagent, quality control, and standard precaution.
Type II
Determine which type of water purity
For qualitative chemistry procedures and for most procedures done in hematology, immunology, microbiology, etc.
Type II
Determine which type of water purity
Glassware washing but not for reagent preparation
Type III
Determine which type of water purity
Used in urinalysis
Type III
Identify which kind of glassware
Usually a low borosilicate glass with low alkali content
High Thermal Resistant or Borosilicate Glass
Identify which kind of glassware
Resistant to high temperature, heat corrosion or thermal shock
High Thermal Resistant or Borosilicate Glass
Identify which kind of glassware
Used when heating or sterilization
High Thermal Resistant or Borosilicate Glass
Most common borosilicate glasses
- Beakers
- Flasks
- Pipettes
Kimax and Pyrex are examples of ____.
High Thermal Resistant or Borosilicate Glass
Identify which kind of glassware
Made by removing all the elements from borosilicate glass
High Silica Glass
Identify which kind of glassware
Has good optical qualities, temperature capabilities, and radiation resistant.
High Silica Glass
Identify which kind of glassware
Used in cuvettes and thermometers
High Silica Glass
Identify which kind of glassware
Used for high precision analytical work and for optical reflectors and mirrors.
High Silica Glass
Identify which kind of glassware
Not used for the type of glassware generally used in the laboratory
High Silica Glass
Identify which kind of glassware
Partially used for strong alkali solution
High Alkali Resistant Glass
Identify which kind of glassware
Often referred as “soft glass” for its thermal resistance is much less than of borosilicate glasses.
High Alkali Resistant Glass
Identify which kind of glassware
Has materials that usually impart red color to the glass that reduce the amount of light passing through the substance inside the glassware.
Low Actinic Glass
Identify which kind of glassware
Used for substances that are particularly sensitive to light such as bilirubin and Vitamin A
Low Actinic Glass
Identify which kind of glassware
Used to manufacture weighting bottles because it develops less static surface changes.
Standard Flint Glass or Soda-Lime Glass
Identify which kind of glassware
Composed of a mixture of the oxides of silicon, calcium, and sodium
Standard Flint Glass or Soda-Lime Glass
Glassware
- High Thermal Resistant or Borosilicate Glass
- High Silica Glass
- High Alkali Resistant Glass
- Low Actinic Glass
- Standard Flint or Soda-Lime Glass
____ is used in light fitters, lamp bulbs, and lightning lenses.
Colored and Opal Glasses
____ have thin metallic oxide, permanently fine-bonded to the surface of the glass.
Coated Glassware
____ are mostly soda-limed, lead, and borosilicate of high optical purity.
Optical Glasses
____ have high thermal resistance, chemical stability and corrosion resistance like borosilicate glasses.
Glass Ceramics (Pyroceram)
____ is useful in preventing transmission of huge energy radiation.
Radiation-Absorbing Glasses
Determine which type of plasticware
A unique group of resins with relatively inert properties.
Polyolefins
Determine which type of plasticware
Unaffected by acids, alkali, salt solutions, and aqueous solutions
Polyolefins
Determine which type of plasticware
Can be autoclaved
Polyolefins
Two Kinds of Polyolefin
- Polypropylene
- Polyethylene
Identify which kind of Polyolefin
More vulnerable to attack by oxidizing agent
Polypropylene
Identify which kind of Polyolefin
Can withstand higher temperature
Polypropylene
Identify which kind of Polyolefin
Used primarily to fabricate bottles, jars, beakers, funnel, pipette connectors, and tubing.
Polyethylene
Identify which kind of Polyolefin
Has two kinds: Coventional and Linear
Polyoethylene
Determine which type of plasticware
Twice as strong as polypropylene and maybe used at temperatures ranging from 100 C - 160 C.
Polycarbonate Resin
Determine which type of plasticware
Unsuitable for use with bases such as amines, ammonia, alkaline and oxidizing agents.
Polycarbonate Resin
Determine which type of plasticware
Dissolved by chlorinated aliphatic and aromatic hydrocarbons
Polycarbonate Resin
Polycarbonate resin can be dissolved by ____.
Chlorinated aliphatic and aromatic hydrocarbons
Determine which type of plasticware
Used extensively in centrifuge tubes and graduated cylinders
Polycarbonate Resin
Determine which type of plasticware
Non-toxic, clear plastic of modified plasticized polyvinyl chloride.
Tygon
Tygon is a modified ____.
plasticized polyvinyl chloride
Determine which type of plasticware
Used to handle most chemicals but should not be subjected to prolonged immersion in aliphatic and aromatic hydrocarbons, ketones, and esters.
Tygon
Determine which type of plasticware
Flexible at 30 C, brittle at 45 C, and resist dry heat to 95 C
Tygon
Tygon is flexible at ____.
30 C
Tygon is brittle at ____.
45 C
Tygon resists dry heat at ____.
95 C
Determine which type of plasticware
Can be steamed, autoclaved, or chemically sterilized.
Tygon
Determine which type of plasticware
Use for manufacturing rubber tubing (ex. WBC and RBC pipette)
Tygon
Determine which type of plasticware
Pure transluscent white and inert to corrosive reagents, nitric acid, boiling chemicals, etc.
Teflon-Fluorocarbon Resin
Determine which type of plasticware
Can resist extreme temperatures ranging from -270 C to 255 C.
Teflon-Fluorocarbon Resin
Determine which type of plasticware
Used in cryogenic experiment or work at temperature over extended periods.
Teflon-Fluorocarbon Resin
Determine which type of plasticware
Used for self-lubricating stopcocks, stirring bars, and bottle cap lines and tubing because of its anti-adhesive properties.
Teflon-Fluorocarbon Resin
Three Major Types of Thermometer
- Liquid-in-glass
- Electronic thermometer or thermistor probe
- Digital thermometer
Which Type of Thermometer?
Use a colored liquid (red or other colors) or mercury.
Liquid-in-glass
Which Type of Thermometer?
Encased in plastic or glass material with a bulb and a graduated stem
Liquid-in-glass
Three types of liquid-in-glass thermometer
- Partial immersion
- Total immersion
- Surface
Which Type of Thermometer?
An accurate, fast-reading electronic thermometer.
Thermistor
Which Type of Thermometer?
Advantages are size and millisecond response time
Thermistor
Vessels holding or transferring liquid are designed to either:
- To Contain (TC)
- To Deliver (TD)
____ do not deliver the same volume when the liquid is transferred into a container.
To Contain (TC)
____ means that the lab ware will deliver the same amount when the liquid is transferred into a container.
To Deliver (TD)
____ is calibrated to hold one exact volume of liquid.
Volumetric Flask
____ is designed to hold different volumes.
Erlenmeyer Flasks and Griffin Beakers
____ are long, cylindrical tubes usually held upright by an octagonal or circular base.
Graduated Cylinder
____ can be used to measure specified volume of liquid, and to measure high degree of accuracy.
Graduated Cylinder
____ are glass or plastic utensils used to transfer liquids.
Pipettes
Pipettes are usually used for volumes of ____ or less.
20 mL
Meniscus line: clear solution
Lower meniscus
Meniscus line: colored solution
Upper meniscus
Kinds of Pipette According to Design
- To Contain (TC)
- To Deliver (TD)
Kinds of Pipette According to Drainage Characteristics
- Blowout
- Self-draining
Kinds of Pipette According to Drainage Characteristics
Has a continuous etched ring or two small, close, continuous rings located near the top of the pipette.
Blowout
Kinds of Pipette According to Drainage Characteristics
The last drop of liquid should be expelled into the receiving vessel.
Blowout
Kinds of Pipette According to Drainage Characteristics
Without markings
Self-draining
Kinds of Pipette According to Drainage Characteristics
Allows the contents of the pipette to drain by gravity
Self-draining
Kinds of Pipette According to Type
- Measuring or Graduated
- Transfer
Measuring or Graduated Pipettes
- Serologic
- Mohr
- Bacteriologic
- Ball, Kolmer, or Kahn
- Micropipette
Which Kind of Measuring/Graduated Pipette?
Has graduation marks to the tip and is generally a blowout pipette
Serologic
Which Kind of Measuring/Graduated Pipette?
Does not have calibration to the tip, and is usually a self-draining pipette
Mohr
What kind of pipette are serologic and Mohr pipettes according to their design?
To Deliver (TD)
Transfer Pipettes
- Volumetric
- Ostwald-Folin
- Pasteur
- Automatic macropipettes or micropipettes
Which Kind of Transfer Pipette?
Has a bulb in the center, and is usually self-draining
Volumetric
Which Kind of Transfer Pipette?
Used for greatest degree of accuracy or precision
Volumetric
Which Kind of Transfer Pipette?
Used for viscous fluids
Ostwald-Folin
Which Kind of Transfer Pipette?
Has a bulb near the tip, and is usually a blowout pipette
Ostwald-Folin
Which Kind of Transfer Pipette?
Has no calibration marks
Pasteur Pipettes
Which Kind of Transfer Pipette?
Pipette with a total holding volume of less than 1 mL
Micropipette
Which Kind of Transfer Pipette?
It may be designed as either a Mohr or Serologic pipette
Micropipette
Which Kind of Transfer Pipette?
To contain pipette, which is calibrated with mercury
Micropipette
Types of Automatic Pipette
- Air displacement
- Positive displacement
- Dispensers/dilutors
Which Type of Automatic Pipette?
Relies on a piston for suction creation to draw the sample into a disposable tip that must be changed after each use.
Air Displacement
Which Type of Automatic Pipette?
Operates by moving the piston in the pipette tip or barrel much like a hypodermic syringe.
Positive Displacement Pipette
Which Type of Automatic Pipette?
Obtain the liquid from a common reservoir and dispense it repeatedly.
Dispensers and dilutor