LABORATORY Flashcards
GRAM STAINING
stain with (?) then gently wash with water.
Use (?) then gently wash with water.
Decolorize with (?).
Counter stain with (?) then gently wash with water.
Crystal Violet for 2 minutes
Lugol’s lodine for 2 minutes
Acid Acetone for 10 to 15 seconds
safranin for 45 seconds to 1 minute
MANNER OF REPORTING:
Smear shows «(?)»«(?)»«(?)»«(?)»«(?)»«(?)»«(?)»
(If present: smear is positive for intracellular/extracellular gram negative diplococci.)
occasional/few/moderate/many
gram’s reaction and/or yeast cells
bacilli/ coccis in singles/pairs/ chains/ clusters
occasional/few/moderate/many
occasional/few/ moderate/many
epithelial cells were also seen
ACID FAST STAINING
Stain with (?) then gently wash with water.
Use (?) for and allow it to steam and then gently wash with water (Do not let the stain boil).
Decolorize with (?).
Counter stain with (?) then gently wash with water.
carbol Fuchsin for 5 minutes
heat
Acid Alcohol for 10 to 15 seconds
Methylene Blue for 30 seconds
ACID FAST MANNER OF REPORTING:
(- or AFB NOT SEEN):
(+/- or DOUBTFUL):
1+:
2+:
3+:
4+:
0 AFB seen per 300 fields
1-2 per 300 fields AFB seen
1-9 per 100 fields AFB seen
1-9 per 10 fields AFB seen
1-9 per Field AFB seen
> 9 per field AFB seen
KOH MOUNT
1. Place [?] on a clean glass slide.
• For soft samples, let it stand for [?].
• In case of nail clippings, dissolve it first in [?] in a small test tube and stand for [?] before placing it on a slide.
2. Place a small amount of specimen and mix.
• Pass the slide [?] over a low flame in a bunsen burner. Do not overheat. Overheating will cause crystals to form.
3. Cover with coverslip.
• Apply gentle pressure using a [?] to disperse the specimen and place in a moist chamber to prevent specimen from drying.
4. Stand for [?].
• Allow the clearing of specimen.
5. Examine and report results.
• Examine under the microscope with reduced light source in [?] and shift to [?].
1-2 drops of 10% KOH
5 minutes
20% KOH ; 20-30 minutes
2-3 times
pencil eraser or forceps
5 minutes
LPO ;
● Maintaining an accurate inventory of all materials in the laboratory. This includes tracking incoming and outgoing materials.
MATERIAL MANAGER
● Ensuring that the supplies are properly stored.
MATERIAL MANAGER
● Identifying any potential shortages in supplies.
MATERIAL MANAGER
● Making sure that all the materials, equipment, and reagents used are back to their proper places.
MATERIAL MANAGER
● Collecting dirty glasswares and materials.
WASHER
● Washing glasswares and other materials used during the laboratory activities.
WASHER
● Sterilizing glasswares using an autoclave.
WASHER
● Storing clean glassware and other materials in a designated area.
WASHER
● Cleaning the laboratory spaces.
HOUSEKEEPER
● Making sure that wastes are properly segregated and disposed.
HOUSEKEEPER
● Turning on the UV lamp 15 minutes before entering and leaving the laboratory.
SANITATION
● Disinfecting the table tops and other laboratory spaces.
SANITATION
● Monitoring and logging the temperature of the room as well as the laboratory equipment in the Microbiology section.
MAINTENANCE
● Turning on the exhaust system upon entering the laboratory and turning it off before leaving.
MAINTENANCE
● Reading and following standard operating procedures (SOPs) for media preparation.
MEDIA PREPARATION
● Mixing contents according to SOPs.
MEDIA PREPARATION
● Sterilizing media using the autoclave.
MEDIA PREPARATION
● Storing media in a cool, dark place.
MEDIA PREPARATION
● Proper dispensing of media.
MEDIA PREPARATION
● Troubleshooting media problems.
MEDIA PREPARATION
MICROBIOLOGY LABORATORY WORKFLOW • SPECIMEN COLLECTION AND TRANSPORT
• PRE-ANALYTICAL TESTING
• GRAM STAINING
• CULTURE & BIOCHEMICAL TESTING
• IDENTIFICATION
• MICROSCOPY
• ANTI-SUSCEPTIBILITY TESTING
• REPORTING
DAY 1
Media Preparation of culture plates
Cooking of biochemical agar
DAY 1
Media Preparation of culture plates
Cooking of biochemical agar
