LAB1. The Microscope Flashcards

1
Q

Instrument equipped with various lenses that is used to magnify objects that are not easily seen by the naked eye

A

Microscope

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2
Q

He was a Dutch scientist who is commonly known as the “Father of Microbiology.”

He made significant contributions to the field of microscopy and the understanding of microscopic organisms.

He is renowned for his development and improvement of the microscope.

A

Anton van Leeuwenhoek

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3
Q

2 basic principles of microscopes (what do they do?)

A
  1. Magnification
  2. Resolution
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4
Q

It means the number of times the image of an object is enlarged compared with the actual size of the object when seen by the unaided eye

A

Magnification

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5
Q

The ability to see objects that are close together distinctly

A

Resolution

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6
Q

What is the Total Magnification Formula?

A

Ocular magnification X Objective magnification = Total Magnification

Ex.
(10x) X (40x) = 400x

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7
Q

What is resolving power of human eye?

A

0.02 mm

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8
Q

What is resolving power of compound light microscope?

A

0.2 micrometers (μm)

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9
Q

What is resolving power of transmission electron microscope?

A

0.2 nanometers (nm)

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10
Q

These types of microscopes are based on the simple principle of light and lens

A

Light Microscope

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11
Q

Light microscope that uses single lens

A

Simple Light Microscope

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12
Q

Light microscope that uses a set of lens or lens system (transmitted light)

A

Compund Light Microscope

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13
Q

Light microscope that uses a set of lens or lens system (reflected light)

A

Dissceting Light Microscope

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14
Q

__________ is an optical instrument which consists of a single lens or magnifying glass set in a frame; it has low magnifying power and produces an enlarge upright image of an object.

A

Simple or single microscope

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15
Q

Light microscope that uses a set of lens or lens system (reflected electron beam)

A

Scanning Electron Microscope

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16
Q

__________ is an optical instrument that can magnify an image up to several hundred times the actual size of an object.

A

Compound microscope

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17
Q

__________ is essentially two microscopes, one for each eye, mounted on a single body for convenient use.

A

Stereoscopic microscope

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18
Q

__________ can magnify an image several thousand times the actual size of a very minute object which cannot be perceived even by the most powerful lenses of an optical microscope.

A

Electron microscope

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19
Q

Light microscope that uses a set of lens or lens system (transmitted electron beam)

A

Transmission Electron Microscope

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20
Q

In light microscopy, objects are measured in __________

A

Micrometers (μm)

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21
Q

1 micrometer (μm) is equal to:

A

1/1000th of 1 mm

22
Q

In electron microscopy, objects are measured in __________

A

Nanometers (nm)

23
Q

1 nanometer (nm) is equal to:

A

1/1000th of 1 μm

24
Q

3 Parts of Compund Microscope (3)

A
  1. Mechanical Parts
  2. Magnifying Parts
  3. Illuminating Parts
25
Q

What is the function of mechanical parts of microscope?

A

Used to support and adjust the parts

26
Q

What is the function of magnifying parts of microscope?

A

Used to enlarge the specimen

27
Q

What is the function of illuminating parts of microscope?

A

Used to provide light

28
Q

Bottom most part of microscope used to support the entire/ lower microscope

A

Base

29
Q

Part above the base that supports the other parts

A

Pillar

30
Q

Allows for tilting of the microscope for convenience of user

A

Inclination Joint

31
Q

Curved/ slanted part which is held while carrying the microscope

A

Arm/neck

32
Q

Platform with a central aperture where the object to be examined is placed

A

Stage

33
Q

Secures the specimen to the stage

A

Stage clips

34
Q

Attached to the arm and bears the objective lens

A

Body tube

35
Q

Cylindrical structure on top of the body tube that holds the ocular lenses

A

Draw tube

36
Q

Rotating disc where the objectives are attached

A

Revolving/ Rotating Nosepiece

37
Q

Lies atop the nosepiece and keeps dusts from settling on the objectives

A

Dust shield

38
Q

Geared to the body tube which elevates or lowers when rotated, bringing the object into approximate focus

A

Coarse adjustment knob

39
Q

A smaller knob for delicate focusing bringing the object into perfect focus

A

Fine adjustment knob

40
Q

Elevates and lowers the condenser to regulate the intensity of light

A

Condenser adjustment knob

41
Q

Lever infront of the condenser and which is moved horizontally to open/close the diaphragm

A

Iris Diaphragm Lever

42
Q

Located beneath the stage and has concave and plane surfaces to gather and diect light in order to illuminate the object

A

Mirror

43
Q

A built-in illuminator beneath the stage that may be used if sunlight is is not preferred or available

A

Electric lamp

44
Q

Regulates the amount of light necessary to obtain a clearer view of the object

A

Iris diaphragm

45
Q

A set of lenses between the mirror and the stage that concentrates light rays on the specimen

A

Condenser

46
Q

Another set of lens found on top of the body tube which functions to furthe rmagnify the image produced by the objective lens. It usually ranges form 5x to 15x

A

Ocular or Eyepiece

47
Q

Metal cyclinders attached below the nosepiece and contains especially ground and polished lenses

A

Objectives

48
Q

Gives the lowest magnification, usually 10x

A

LPO or Low Power Objective

49
Q

Gives higher magnification, usually 40x or 43x

A

HPO or High Power Objective

50
Q

Gives the highest magnification usually 97x or 100x and is used with either cedarwood oil or synthetic oil

A

OIO or Oil Immersion Objective

51
Q

Can magnify up to 4x

A

Scanner objective