Lab week 5 Flashcards
Differential stain process that allows you to classify bacteria as either Gram positive or negative
Gram stain
Distinguishes organisms based on their interactions with multiple stains
Differential staining
A substance used to set or stabilize stains or dyes. In this experiment: Gram’s iodine
Mordant
What cultures are we working with in exercise 6?
Staphylococcus epidermidis
Escherichia coli
Bacillus subtilis
What primary stain are we using in exercise 6?
Crystal violet
Media containing only a single kind of microbe
pure culture
in the 1970s, who had limited success in obtaining pure cultures by performing serial dilutions?
Joseph Lister
In 1880, who was able to obtain pure cultures through making solid media where microbes could be separated by dilution?
Robert Koch
The growth of one specific bacteria
isolated colony
Has several isolated colonies of different types of bacteria
mixed culture
What are the 3 techniques used to get isolated colonies from mixed cultures that we will be implementing in exercise 7?
- Streak plate
- Spread plate
- pour plate
What technique is when a sterile loop is used to spread a sample over the surface of an agar plate?
streak plate
What technique is a small amount of previously diluted sample is spread on the surface of an agar plate using a sterile, bent glass rod
spread plate
what is the technique in which a small amount of sample is mixed with melted agar and poured into sterile, empty petri dishes. After incubation, growth is visble as colonies in and on the agar of the pour plate.
Pour plate
In exercise 7, what general, complex medium will we be using?
Tryptose
