lab tests part two

Question 1

for the MSA plate test, how does it differentiate between mannitol fermentation? if the results are positive what does it look like? negative?

Answer 1

if the results are positive then there is yellow growth.
if the results are negative, there is no change/ no growth

Question 2

what kind of bacteria grown on EMB plates?

Answer 2

gram negative enteric bacteria

Question 3

in an EMB plate, if lactose and sucrose are present there is a high amount of fermentation so you would get a_______ _________ ________

Answer 3

green metallic sheen

Question 4

in an EMB plate one sugar shows? no sugars show?

Answer 4

pink, no change

Question 5

what is meant by reactive oxygen species?

Answer 5

means there are side reactions from electron transport carriers

Question 6

what proteins participate in side reactions?

Answer 6

iron sulfer proteins, flavoproteins, quinones

Question 7

superoxide

Answer 7

quickly taken care by enzymes

Question 8

what enzymes participate in superoxide reactions?

Answer 8

superoxide dismotase, peroxidase, catalase

Question 9

what is used in the catalase test?

Answer 9

hydrogen peroxide

Question 10

what does a positive result suggest in the catalase test?

Answer 10

bubbles, the bacteria produces catalase

Question 11

what kind of bacteria is used in the catalase test?

Answer 11

bacteria that is dependent on oxygen (aerobes/facultative anaerobes)

Question 12

what does the oxidase test do?

Answer 12

looks at the composition of the ETC

Question 13

what key component does the oxidase test look for?

Answer 13

cytochrome c

Question 14

what is cytochrome c?

Answer 14

peripheral membrane protein

Question 15

what is added to oxidase test to look for cytochrome c?

Answer 15

tetramethyl-p-phenylenediamine

Question 16

T/F tetramethyl-p-phenylenediamine is clear in its induced state

Answer 16

true

Question 17

in the oxidase test if tetramethyl-p-phenylenediamine can exchage electrons with cytochrome c then the test should be what color?

Answer 17

dark purple/blue

Question 18

the oxidase test is most helpful with?

Answer 18

pseudomonas

Question 19

what are API strips?

Answer 19

standardized and miniaturized version of specific biochemical tests.

Question 20

how many test are on the API strips?

Answer 20

21 tests total but 20 on the strip

Question 21

what does the API strip not look for?

Answer 21

enterobacteriaceae

Question 22

what bacteria does the API strips test for mostly?

Answer 22

gram negative bacteria

Question 23

what is the layout of the API strip?

Answer 23

7 groups of 3 tests

Question 24

what are the individual tests called on the API strip?

Answer 24

Cubules

Question 25

what do you inoculate the dehydrated media with to rehydrate the media in an API strip?

Answer 25

broth