lab tests part two Flashcards
for the MSA plate test, how does it differentiate between mannitol fermentation? if the results are positive what does it look like? negative?
if the results are positive then there is yellow growth.
if the results are negative, there is no change/ no growth
what kind of bacteria grown on EMB plates?
gram negative enteric bacteria
in an EMB plate, if lactose and sucrose are present there is a high amount of fermentation so you would get a_______ _________ ________
green metallic sheen
in an EMB plate one sugar shows? no sugars show?
pink, no change
what is meant by reactive oxygen species?
means there are side reactions from electron transport carriers
what proteins participate in side reactions?
iron sulfer proteins, flavoproteins, quinones
superoxide
quickly taken care by enzymes
what enzymes participate in superoxide reactions?
superoxide dismotase, peroxidase, catalase
what is used in the catalase test?
hydrogen peroxide
what does a positive result suggest in the catalase test?
bubbles, the bacteria produces catalase
what kind of bacteria is used in the catalase test?
bacteria that is dependent on oxygen (aerobes/facultative anaerobes)
what does the oxidase test do?
looks at the composition of the ETC
what key component does the oxidase test look for?
cytochrome c
what is cytochrome c?
peripheral membrane protein
what is added to oxidase test to look for cytochrome c?
tetramethyl-p-phenylenediamine
T/F tetramethyl-p-phenylenediamine is clear in its induced state
true
in the oxidase test if tetramethyl-p-phenylenediamine can exchage electrons with cytochrome c then the test should be what color?
dark purple/blue
the oxidase test is most helpful with?
pseudomonas
what are API strips?
standardized and miniaturized version of specific biochemical tests.
how many test are on the API strips?
21 tests total but 20 on the strip
what does the API strip not look for?
enterobacteriaceae
what bacteria does the API strips test for mostly?
gram negative bacteria
what is the layout of the API strip?
7 groups of 3 tests
what are the individual tests called on the API strip?
Cubules
what do you inoculate the dehydrated media with to rehydrate the media in an API strip?
broth
