Lab test 1 BT Flashcards
Photography shows an electrophoresis of PCR amplification products seperated on polyacrylamide gel. It is an analysis of gene fragment of angiotensen converting enzyme. Each of the alleles may contain a deletion (length 190 base pairs) or insertion (length 480 base pairs). Which parts show homozygous genotype with deletion (DD)?
c. C (picture)
Reverse transcription polymerase chain reaction is used to
b)Study of Gene expression
Restriction endonucleases cleave DNA at the site of a palindrome, Choose the DNA sequence which can be a site of action for this enzymes
c. 5’-CAATTG- 3’
Choose the correct statement concerning real-time PCR
c. in this method the cycle number at which the fluorescence becomes detectable over a defined threshold is indirectly proportional to the number of copies of the orinigal template
Which of the following sentences about electrophoresis is NOT CORRECT?
c. During electrophoresis nucleic acids migrate towards cathode
Choose the correct sequence of steps of PCR-RFLP analysis
a. DNA isolation, PCR reaction, Digestion with restriction enzyme, separation on gel
Choose the correct sentence concerning STR:
c. Each STR can occur in 5 to 15 different lengths of which one individual posesses only one or two alleles.
During the DNA isolation from blood, the isopropanol can be used for:
a. precipitation of the DNA
Choose the correct statement concerning RNA:
c. pure, intact RNA is a critical requirement for microarray analysis
Which of the following molecular tests can be used for quantification of hepatitis B serum sample
a. real-time PCR
At which cycle of PCR reaction first double strand product with a length equal to the distance between the two primers is obtained?
b)third
qPCR reaction components include:
c) cDNA template, SYBR green 1, 2 primers, dNTPs, heat tolerant DNA polymerase
During the DNA isolation from blood, the UREA can be used for:
c) Deproteinization
Reverse transcription PCR produced DNA fragment with sequence; TGACTGA. The nucleotide sequence of RNA was:
b) ACUGACU
On agarose gel, after electrophoresis, to make the separated fragment visible we can see:
d) none of the above ANSWERS IS: ethidium bromide
sodium azide is used for prservation of agarose solutions and to avoid growth of microorganisms (0.1% sodium azide)
Which of the following sentences about electrophoresis is correct:
b) during electrophoresis nucleic acids migrate towards the anode
Choose the false sentence about restriction endonuclease:
d) it cleaves the single strand DNA
Which molecular method is used in forensic medicine to identify individals:
c) PCR-STR
DNA cannot be isolated from:
c) Erythrocytes
Which anti-coagulent is preffered for DNA isolation:
a) EDTA
Choose the correct sequence of steps for PCR, repeated 20 to 40 times:
b) Denaturation, hybridiaztion, elonagtion
Mobility of DNA during electrophoresis does not depend on:
c) charge of DNA
Choose the incorrect statement of Real-Time PCR:
c) Real-Time PCR and reverse transcription polymerase chain reaction (RT-PCR) is the same method
In short repeated sequences, (STR), the most common number of bases repeated is:
d) 4
Reverse Transcription Polymerase Chain Reaction (RT-PCR), is used for:
a) Identification of RNA viruses (Ex. HIV)
Which technology should be used to detect single known mutations in DNA?
b) PCR-RFLP
Short repeated sequences (STR) method that uses highly polymorphic regions of DNA may be used to:
d) DNA fingerprinting
