Lab test 1 BT

Question 1

Photography shows an electrophoresis of PCR amplification products seperated on polyacrylamide gel. It is an analysis of gene fragment of angiotensen converting enzyme. Each of the alleles may contain a deletion (length 190 base pairs) or insertion (length 480 base pairs). Which parts show homozygous genotype with deletion (DD)?

Answer 1

c. C (picture)

Question 2

Reverse transcription polymerase chain reaction is used to

Answer 2

b)Study of Gene expression

Question 3

Restriction endonucleases cleave DNA at the site of a palindrome, Choose the DNA sequence which can be a site of action for this enzymes

Answer 3

c. 5’-CAATTG- 3’

Question 4

Choose the correct statement concerning real-time PCR

Answer 4

c. in this method the cycle number at which the fluorescence becomes detectable over a defined threshold is indirectly proportional to the number of copies of the orinigal template

Question 5

Which of the following sentences about electrophoresis is NOT CORRECT?

Answer 5

c. During electrophoresis nucleic acids migrate towards cathode

Question 6

Choose the correct sequence of steps of PCR-RFLP analysis

Answer 6

a. DNA isolation, PCR reaction, Digestion with restriction enzyme, separation on gel

Question 7

Choose the correct sentence concerning STR:

Answer 7

c. Each STR can occur in 5 to 15 different lengths of which one individual posesses only one or two alleles.

Question 8

During the DNA isolation from blood, the isopropanol can be used for:

Answer 8

a. precipitation of the DNA

Question 9

Choose the correct statement concerning RNA:

Answer 9

c. pure, intact RNA is a critical requirement for microarray analysis

Question 10

Which of the following molecular tests can be used for quantification of hepatitis B serum sample

Answer 10

a. real-time PCR

Question 11

At which cycle of PCR reaction first double strand product with a length equal to the distance between the two primers is obtained?

Answer 11

b)third

Question 12

qPCR reaction components include:

Answer 12

c) cDNA template, SYBR green 1, 2 primers, dNTPs, heat tolerant DNA polymerase

Question 13

During the DNA isolation from blood, the UREA can be used for:

Answer 13

c) Deproteinization

Question 14

Reverse transcription PCR produced DNA fragment with sequence; TGACTGA. The nucleotide sequence of RNA was:

Answer 14

b) ACUGACU

Question 15

On agarose gel, after electrophoresis, to make the separated fragment visible we can see:

Answer 15

d) none of the above ANSWERS IS: ethidium bromide

sodium azide is used for prservation of agarose solutions and to avoid growth of microorganisms (0.1% sodium azide)

Question 16

Which of the following sentences about electrophoresis is correct:

Answer 16

b) during electrophoresis nucleic acids migrate towards the anode

Question 17

Choose the false sentence about restriction endonuclease:

Answer 17

d) it cleaves the single strand DNA

Question 18

Which molecular method is used in forensic medicine to identify individals:

Answer 18

c) PCR-STR

Question 19

DNA cannot be isolated from:

Answer 19

c) Erythrocytes

Question 20

Which anti-coagulent is preffered for DNA isolation:

Answer 20

a) EDTA

Question 21

Choose the correct sequence of steps for PCR, repeated 20 to 40 times:

Answer 21

b) Denaturation, hybridiaztion, elonagtion

Question 22

Mobility of DNA during electrophoresis does not depend on:

Answer 22

c) charge of DNA

Question 23

Choose the incorrect statement of Real-Time PCR:

Answer 23

c) Real-Time PCR and reverse transcription polymerase chain reaction (RT-PCR) is the same method

Question 24

In short repeated sequences, (STR), the most common number of bases repeated is:

Answer 24

d) 4

Question 25

Reverse Transcription Polymerase Chain Reaction (RT-PCR), is used for:

Answer 25

a) Identification of RNA viruses (Ex. HIV)

Question 26

Which technology should be used to detect single known mutations in DNA?

Answer 26

b) PCR-RFLP

Question 27

Short repeated sequences (STR) method that uses highly polymorphic regions of DNA may be used to:

Answer 27

d) DNA fingerprinting