Lab Tech

Question 1

Direct Selection

Answer 1

A form of Mutant Selection where cells are inoculated into a selective agar medium that only promotes the growth of the mutatnt

Question 2

Indirect Selection

Answer 2

When the non-mutant is a prototroph but the mutant is a auxotroph, this method is used to isolate the mutant

Question 3

Steps for Replica Plating

Answer 3

1. Sterilize velvet and press the master plate onto it
2. inoculate a minimal agar medium and a nutrient agar medium
3. observe the colonies that didn’t grow on the glucose-salt agar– those are the mutations

Question 4

Penicillin Enrichments

Answer 4

Selectively kills prototrophs before replica plating to increase the proportion of auxotrophic mutants