Lab Stuff

Question 1

Haemophilus media

Answer 1

Chocolate Agar
Factor X (hematin) and V (NAD)

Question 2

Cold Agglutinin

Answer 2

IgM Abs that agglutinate RBCs at 4C

Question 3

Neisseria gonorrhoeae Culture

Answer 3

Don’t refrigerate before analysis

• can survive @ 20C for 6-12h
• place rectal swabs on Thayer-Martin

Question 4

Bordatella purtussis media

Answer 4

Bordet-Gengou agar

Question 5

Blood Culture

Answer 5

Take prior to abx tx, take 2-3 sets (1 aerobic + 1 anaerobic = 1 set) and take them from different sites

• disinfect with alcohol and betadine or chlorhexadine too
• don’t use iodine for children <2yo

Question 6

C. diphtheriae selective agar

Answer 6

Tellurite agar

Question 7

MacConkey’s Agar

Answer 7

Selective and Differential (inhibits growth of G+ve)

Question 8

Neisseria from sites w/ nml flora (selective)

Answer 8

Thayer-Martin selective medium

*chocolate plus other stuff

Question 9

Oleic acid broth

Answer 9

Cultures M. tuberculosis

Question 10

Legionella Selective media

Answer 10

Buffered charcoal yeast extract (BCYE) agar

Question 11

Vibrio cholerae (selective, alkaline)

Answer 11

Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS)

Question 12

Mycobacterium Selective media

Answer 12

Lowenstein-Jensen agar

Question 13

UA

Answer 13

Bacterial growth doubles every 30 minutes, refrigerate urine sample within 1 hour
- Symptomatic UTI: >10^5 CFU/mL of urine

Question 14

Blood Agar

Answer 14

Streptococcus

Question 15

Tellurite Agar

Answer 15

Corynebacterium (differential)

Question 16

What is MIC?

Answer 16

Minimal Infectious Concentration

- used to determine concentration of antimicrobial an organism is sensitive to

Question 17

Vibrio cholera media

Answer 17

TCBS (sucrose is differentiating bc only V. cholera is sucrose +ve)

Question 18

Staph aureus medium

Answer 18

Mannitol salts agar (confirm w/ coagulase +ve test)