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Bacteriology 3rdYR 1stSem Prelims > (LAB) Specimen Collection > Flashcards

(LAB) Specimen Collection Flashcards

1
Q

When should specimen be collected

A

Acute phase of illness

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2
Q

What to use when patient is on antimicrobial

A

Thiol Broth

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3
Q

Sulfonamide can be neutralize by addition of?

A

P-amino benzoic acid

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4
Q

Penicillin can be counteracted by adding?

A

Penicillinase

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5
Q

Components of Bacto-Penase Broth

A

Brain Heart Infusion
Penicilinase
P-amino benzoic acid

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6
Q

Preservative for Urine

A

Boric Acid

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7
Q

Preservative for stool

A

Polyvinyl alcohol & Buffered Formalin

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8
Q

Not recommended for viral specimens

A

Calcium Alginate

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9
Q

Urine, stool, sputum, swabs, and viral specimens should be stored at what temperature?

A

Refrigerator Temp (4C)

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10
Q

CSF should be stored at what temperature

A

Incubator Temp (35C)

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11
Q

Specimens for anaerobic culture, sterile body fluid, genital specimen and swabs (ear and eye)
are held in what temperature?

A

Room Temp (22 - 25C)

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12
Q

Serum should be kept at what temp

A

-20C

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13
Q

Tissue specimens and Fecal specimens should be stored at what temp

A

-70C

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14
Q

Specimens that should be Plated ASAP

A
  • Body fluids
  • Bone specimens
  • Gastric Biopsy
  • Suprapubic aspirate of urine
  • Prostatic Specimens
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15
Q

Specimen that can be stored indefinitely at room temp

A

Hair, Nail, or Skin scrapings

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16
Q

Specimens that should be immediately stored at 35C

A
  • Blood
  • Bone marrow specimens
  • Corneal scrapings
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17
Q

Specimen that can be stored for 6hrs at 35C

A

CSF

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18
Q

Specimen that can be stored for 72hrs at 4C

A

Rectal swab
Stool culture

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19
Q

Maintains the integrity of the specimen from the time of specimen collection until
laboratory processing of the sample

A

Transport Medium

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20
Q

Transport medium for stool pathogens

A

Cary Blair

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21
Q

Transport medium for virus transport media

A

Stuart’s

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22
Q

Transport medium for Respiratory specimen

A

Amies

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23
Q

Transport medium for Neisseria

A

Transgrow / Cary Blair

24
Q

Transport medium for Streptococcus agalactiae

A

Cary Blair

25
Q

Level 1 or Critical / Invasive Specimens

A
  • Amniotic Fluid
  • Blood
  • Brain
  • CSF
  • Serous Fluids
26
Q

Level 2 or Unpreserved Specimens

A
  • Bone
  • Feces
  • Sputum
  • Tissue
  • Other Body Fluids
27
Q

Level 3 or Quantitation Required Specimens

A
  • Catheter Tip
  • urine
  • Tissue
28
Q

Level 4 or Preserved Specimens

A
  • Urine
  • Feces
  • Swabs in holding media
29
Q

Blood should be drawn when

A

Febrile Episode (30 - 45mins before fever spikes)

30
Q

Concentration of SPS should not exceed ______

A

0.025% w/v

31
Q

What should be added to prevent interference in blood

A

1% gelatin

32
Q

Signs of growth in blood

A

Hemolysis
Gas bubbles
Turbidity

33
Q

Ideal manner of collection for blood culture

A

Butterfly Infusion

34
Q

How is CSF collected

A

Lumbar Tap / Spinal Tap

35
Q

Purposes of the 4 containers for CSF

A

1st - ClinChem/Sero
2nd - Micorbio for culture
3rd - Clin Hema for cell count
4th - Additional tests for Micobio

36
Q

Specimen of choice for detecting GIT pathogens

A

Stool

37
Q

4 samples from Respiratory Tract

A

Throat Swab
Nasal Swab
Nasopharyngeal Swab
Sputum

38
Q

How many morning specimens for sputum should be collected

A

3 (1hr intervals)

39
Q

When should Sputum be collected

A

Morning (more concentrated)

40
Q

Used to determine the cause of vaginitis, urethritis, cervicitis as well as childbirth infections

A

Genitourinary Specimens

41
Q

Often used to determine STIs

A

Genitourinary Specimens

42
Q

Most fastidious organisms

A

Genitourinary Specimens

43
Q

Culture Method for Genitourinary Specimens

A

Chocolate Agar Plate

44
Q

Transport medium for Genitourinary Specimens

A

Jembec / Gono Pak

45
Q

How many swab must be collected for Genitourinary Specimens

A

2 (1 for gram staining, 1 for culture)

46
Q

Results from animal or human bites, burns, ulcers and traumatic
wounds

A

Exogenous wound infection

47
Q

Attribute to bacterial sources within the patient

A

Endogenous wound infection

48
Q

5 Types of Culture Media

A
  • Nonselective
  • Selective
  • Differential
  • Enriched Media
  • Enrichment Broth
49
Q

Culture Media selections

A

Blood Agar Plate
Chocolate Agar plate
MacConkey Agar

50
Q

Incubation Temp

A

35 - 37C

51
Q

Routine Incubation Time

A

48 - 77hrs

52
Q

Incubation Time for Anaerobes and Broth

A

5 - 7 days

53
Q

Use of cotton swab may release what

A

Toxic Fatty Acids

54
Q

3 different swab equipment that can be used for specimen collection for Bacterial Culture

A

Calcium Alginate
Dacron
Rayon

55
Q

3 way to get urine sample

A

Midstream clean catch
Catheterized
Suprapubic aspirate

Bacteriology 3rdYR 1stSem Prelims (6 decks)

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