(LAB) Biochemical Methods Flashcards
Differentiates Staphylococcus from Streptococcus
Catalase Test
Determines the presence of bacterial Cytochrome C oxidase
Oxidase Test
Anaerobic breakdown of carbohydrates resulting often in the production of large amounts if organic acids
Carbohydrate Fermentation Test
Determines the ability of an organism to ferment specific carbohydrate that is incorporated in a basal medium
Broth Fermentation Test
Catalyzes ONPG to Galactose + O-Nitrophenyl
Beta-Galactosidase
Used to extract Indole
Ehrlich’s Xylene
Action of Decarboxylase Enzyme
Remove carboxyl group of amino acid
Results in formation of dark green color
10% Ferric Chloride
Purpose of ONPG
Detects presence of Late or Slow Lactose Fermenting Strains
Produces Acetoin as intermediate product
Voges-Proskauer Test
Color of H2S+ in LIA test
Black
Gas present in butt means
Presence of Hydrogen Sulfide
Positive bacteria in Phenylalanine Deaminase Test
Proteus
Providencia
Morganella
A/A, H2S+
(SPC)
Salmonella
Proteus
Citrobacter
Color of K/A in TSI
Red Slant / Yellow Butt
