Lab Review - Lab Tests Flashcards
Generates a complete CBC using EDTA whole blood
0.5-1.0 mL of blood needed
Uses laser flow cytometry
Cells are analyzed on 5 perameters
Lasercyte
5 perameters Lasercyte tests for with blood cells
lobularity, nucleus:cytoplasm ratio, cellular volume, cellular density, and granularity
Tests organ functions using dry slide technology
12 chemical tests simultaneously
Serum or plasma
VetTest Chemistry Analyzer
The preferred sample for the VetTest in what kind of tube?
Plasma from a heparin tube (green top)
Two color boxes for reagent slides
Where should they be stored?
Green and purple; freezer
Reagent slides need to be used within how many minutes of opening the foil packet?
15 minutes
With these samples, you must allow the blood to clot before centrifuging. How long does it take to clot?
Serum; 20-30 minutes
When centrifuging a serum sample, how long should it be spun?
10-20 minutes
Why must you use a lithium-heparin tube for the VetTest?
Ca in the blood binds to EDTA
2 Kidney tests performed by the VetTest
BUN and CREA
Increases with renal dz, starvation, and dehydration
BUN
Blood Urea Nitrogen
Accumulation of metabolic waste in the blood (urea, specifically)
azotemia
Byproduct of protein metabolism
Blood Urea Nitrogen (BUN)
Byproduct of muscle metabolism
Creatinine (CREA)
Used in conjunction with BUN test to diagnose kidney dz
CREA
Clinical signs of kidney dz are seen after how much of the kidney is compromised?
75%
Clinical signs are only seen after the liver has lost how much of it’s functioning ability?
80%
This organ has regenerative capabilities
Liver
Liver specific
Created by hepatocytes
Increased levels present with liver dz, trauma to the liver, and toxins
SGPT
AKA ALT
Not liver specific
Synthesized in liver and cardiac and skeletal muscles
SGOT
AKA AST
The byproduct of hemoglobin metabolization
Not liver specific
Increases seen with hepatic lipidosis, hemolytic anemia, hyperthyroidism, and pancreatitis
Billirubin (TBIL)
Main protein of blood plasma made by liver cells
Decrease is more common than increase; decreases can be caused by heartworm dz, ehrlichia, and IBD
Levels below 1.0g/dL are dangerously low
Albumin (ALB)
Pancreatic function tests include
AMYL, LIPA, and GLU
Breaks down starches and glycogen in sugars
Increases can be seen with renal failure or peritonitis
Acute or chronic pancreatitis can cause this as well
Amylase (AMYL)
Assists in breaking down fatty acids
Values are usually parallel to amylase values
Increases seen with pancreatitis, liver and renal dz
Lipase (LIPA)
Evaluates insulin production
Blood must be centrifuged immediately because this binds to RBCs and decreases by 10% per hour
Stress can increase values
Below 60 mg/dL is dangerously low
Glucose (GLU)
A patient testing for their glucose levels should fast for how long before the test?
16-24 hrs
Plays important role in steroid production and levels are helpful in diagnosing hypothyroidism and Cushing’s dz
Chlesterol (CHOL)
Used to determine the hydration levels of a patient
Total Protein (TP)
NOT AN ELECTROLYTE
Most abundant MINERAL in the body
Important role in muscle contraction and blood clotting
Calcium (Ca)
When combined with phosphorus, this creates the material that forms dense, hard teeth and bones
Calcium
Calcium phosphate
Dietary mineral required in high amounts
Excessive amounts will accelerate the progression of renal failure
Phosphorus
Machine used to monitor electrolytes
Uses whole blood, serum, or plasma
35 second results
VetLyte analyzer
Most abundant cation in the body
Maintains osmotic pressure
Acid/base balance
Transmits nerve impulses
Sodium (Na+)
Major intracellular cation in blood
Maintains osmotic pressure
Acid/base balance
Aids in proper nerve and muscle action
Potassium (K)
Maintains cellular integrity
Important in maintaining correct water balance
Chloride (Cl)
Performs PT and PTT tests
Fresh or citrated whole blood
Important information about intrinsic and extrinsic clotting factors and fibrinogen
Coag Dx Analyzer
PT means
ability to clot
PTT
clotting disorder
Evaluates oxygenation and pH balance within the body
Whole blood, serum, or plasma in a single disposable cassette
ARTERIAL BLOOD
VetStat Electrolyte and Blood Gas Analyzer
Two types of alkalosis and acidosis
respiratory and metabolic